Reagent Kits

form:Solution in bottle , components:WST-8

form: Solution (EDTA-Free,100× in DMSO) , components: AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A , specificity of inhibition: Serine proteases, Aminopeptidases, Cysteine proteases, Aspartic proteases

form:Solution (100× in ddH2O) , components:Sodium Fluoride,Sodium Orthovanadate,Imidazole etc. , specificity of inhibition:Acid Phosphatase, Alkaline phosphatase, PTPs, ATPases

form:2 Tube Solution (100×) , components:Each Tube has different components , specificity of inhibition:Each Tube has different specificity of inhibition

form:Solution (100× in DMSO) , components:(-)-p-Bromotetramisole oxalate, Cantharidin, Microcystin LR , specificity of inhibition:Alkaline phosphatases, Ser/Thr phosphatases, Acid and PP1 and PP2A

form: Solution (100× in 70% DMSO) , components: Trichostatin A, Sodium Butyrate, Nicotinamide, EX-527 , specificity of inhibition: Class I/II/III HDAC

form: Solution in bottle , components: 1% NP-40, 0.25% deoxycholate

form: Solution in bottle , components: 1% NP-40, 0.5% deoxycholate, 0.1% SDS

form: Solution in bottle , components: 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS

The Bicinchoninic acid (BCA) assay is a stable, sensitive and highly compatible method for the determination of protein concentration. It is commonly used for quantifying the total amount of proteins after extraction. The detection mechanism of BCA assay involves two reations. The first reation is based on the biruet reaction, in which the protein chelates with copper (II) ion in an alkaline environment and reduces the copper (II) to copper (I), forming a blue complex. The second reaction is the chelation of BCA with the cuprous ion, resulting in an intense purple color. The purple colored reaction product is formed by the chelation of two molecules of BCA with one cuprous ion. The complex exhibits a strong linear absorbance at 562 nm with increasing protein concentrations.

Penicillin-streptomycin-amphotericin B solution, is a commonly used solution to reduce bacterial and fungal contamination in cell culture media. Penicillin can directly interfere with the synthesis of bacterial cell wall and indirectly alter the cell wall by triggering enzymatic reactions. Streptomycin can bind to the 30S subunit of the bacterial ribosomal and inhibit the synthesis of bacterial protein, ultimately leading to the death of susceptible bacteria. The combination of penicillin and streptomycin exhibits effective antibacterial activity against both gram-positive and gram-negative bacteria. Amphotericin B exerts its antifungal effect by binding to ergosterol on the bacterial cell membrane, which will damage membrane, lead to the increase of permeability, leakage of intracellular substances and the disruption of normal metabolism. TargetMol's penicillin-streptomycin-amphotericin B solution (100×) is a sterile, filtered and ready-to-use solution for cell culture. This solution contains 10,000 units/mL of penicillin G sodium salt, 10 mg/mL of streptomycin, and 25 μg/mL of amphotericin B, prepared in 0.85% NaCl. It is recommended for use in cell culture application at 100 units/mL of penicillin, 0.1 mg/mL of streptomycin and 0.25 μg/mL of amphotericin B. Dilute the product 100-fold for use.

Penicillin-streptomycin solution, also known as Pen-Strep, is the most commonly used combined antibiotic solution against both gram-positive and gram-negative bacteria. Penicillin can directly interfere with the synthesis of bacterial cell wall and indirectly alter the cell wall by triggering enzymatic reactions. Streptomycin can bind to the 30S subunit of the bacterial ribosome, and inhibit the synthesis of bacterial protein, ultimately leading to the death of susceptible bacteria. The solution is used to supplement cell culture media to control bacterial contamination. TargetMol's penicillin streptomycin solution (100x) is a sterile, filtered and ready-to-use antibiotic solution for cell culture. This solution contains 10,000 units/mL of penicillin and 10 mg/mL of streptomycin, prepared in 0.9% NaCI. It is recommended for use in cell culture application at 100 units/mL of penicillin and 0.1 mg/mL of streptomycin. Dilute the product 100-fold for use.

TargetMol's GFP Tag Immunomagnetic Beads specifically bind to proteins with the GFP tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.

TargetMol Protein A/G Immunomagnetic Beads enables Protein A/G to cover superparamagnetic microspheres with high density by utilizing bio-nano technology. Protein A/G Immunomagnetic Beads can be used for the immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is suitable for antigen samples derived from cell lysates, cell culture supernatants, serum, ascites, and other sources.

TargetMol's HA Tag Immunomagnetic Beads specifically bind to proteins with the HA tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.

TargetMol's MYC Tag Immunomagnetic Beads specifically bind to proteins with the MYC tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.

TargetMol's Flag Tag Immunomagnetic Beads specifically bind to proteins with the DYKDDDDK (Flag) tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.

TargetMol's GST Tag Immunomagnetic Beads specifically bind to proteins with the GST tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.

TargetMol's His Tag Immunomagnetic Beads specifically bind to proteins with the His tag, which can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is suitable for antigen samples from cell lysates, cell culture supernatants, serum, ascites, etc.

TargetMol Protein A/G Immunomagnetic Beads enables Protein A/G to cover superparamagnetic microspheres with high density by utilizing bio-nano technology. Protein A/G Immunomagnetic Beads can be used for the immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. The Protein A/G Immunoprecipitation Kit comes with prepared buffers to provide a relatively better condition for immunoprecipitation and to enhance the stability. This product is suitable for antigen samples derived from cell lysates, cell culture supernatants, serum, ascites, and other sources.

TargetMol Protein A Immunomagnetic Beads enables Protein A to cover superparamagnetic microspheres with high density by utilizing bio-nano technology. Protein AImmunomagnetic Beads can be used for the immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. The Protein A Immunoprecipitation Kit comes with prepared buffers to provide a relatively better condition for immunoprecipitation and to enhance the stability. This product is suitable for antigen samples derived from cell lysates, cell culture supernatants, serum, ascites, and other sources.

The streptavidin–biotin (SA-Biotin) system is known to have a very high affinity (Kd= 10^-15) and specificity, and is widely used in biochemical field.  TargetMol Mag Beads Streptavidin (3 μm) covalently connects SA to the beads surface through protein-protein coupling method. This enables efficient binding of biotinylated antibodies, nucleic acids, and proteins. The product combines superparamagnetic advantages with unique features including uniform size and regular morphology, facilitating the rapid capture of target molecules and magnetic separation. This product can be used with automated equipment for high-throughput technology.

The streptavidin–biotin (SA-Biotin) system is known to have a very high affinity (Kd= 10^-15) and specificity, and is widely used in biochemical field.  TargetMol Mag Beads Streptavidin (2.8 μm) covalently connects SA to the beads surface through protein-protein coupling method. This enables efficient binding of biotinylated antibodies, nucleic acids, and proteins. The product combines superparamagnetic advantages with unique features including uniform size and regular morphology, facilitating the rapid capture of target molecules and magnetic separation. This product can be used with automated equipment for high-throughput technology.

The streptavidin–biotin (SA-Biotin) system is known to have a very high affinity (Kd= 10^-15) and specificity, and is widely used in biochemical field.  TargetMol Mag Beads Streptavidin (2 μm) covalently connects SA to the beads surface through protein-protein coupling method. This enables efficient binding of biotinylated antibodies, nucleic acids, and proteins. The product combines superparamagnetic advantages with unique features including uniform size and regular morphology, facilitating the rapid capture of target molecules and magnetic separation. This product can be used with automated equipment for high-throughput technology.

The streptavidin–biotin (SA-Biotin) system is known to have a very high affinity (Kd= 10^-15) and specificity, and is widely used in biochemical field.  TargetMol Mag Beads Streptavidin (1 μm) covalently connects SA to the beads surface through protein-protein coupling method. This enables efficient binding of biotinylated antibodies, nucleic acids, and proteins. The product combines superparamagnetic advantages with unique features including uniform size and regular morphology, facilitating the rapid capture of target molecules and magnetic separation. This product can be used with automated equipment for high-throughput technology.

The streptavidin–biotin (SA-Biotin) system is known to have a very high affinity (Kd= 10^-15) and specificity, and is widely used in biochemical field.  TargetMol Mag Beads Streptavidin (300 nm), Sterile covalently connects SA to the beads surface through protein-protein coupling method. This enables efficient binding of biotinylated antibodies, nucleic acids, and proteins. The product combines superparamagnetic advantages with unique features including uniform size and regular morphology, facilitating the rapid capture of target molecules and magnetic separation. This product can be used with automated equipment for high-throughput technology.

The streptavidin–biotin (SA-Biotin) system is known to have a very high affinity (Kd= 10^-15) and specificity, and is widely used in biochemical field.  TargetMol Mag Beads Streptavidin (5 μm) covalently connects SA to the beads surface through protein-protein coupling method. This enables efficient binding of biotinylated antibodies, nucleic acids, and proteins. The product combines superparamagnetic advantages with unique features including uniform size and regular morphology, facilitating the rapid capture of target molecules and magnetic separation. This product can be used with automated equipment for high-throughput technology.

TargetMol His-tag Protein Purification Magrose Beads NTA-Nickel is a new type of superparamagnetic functional material designed for efficient and rapid purification of His-tag proteins. It can directly extract high-purity target proteins from biological samples in one step using magnetic separation method, which greatly simplifies the purification process and improves the efficiency. The method is suitable for scientific research and industrial areas to facilitate the purification of his-tag protein. The His-tag protein purification beads, based on magnetic agarose microspheres, activate and couple iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA). Then, they chelate Nickel and Cobalt ions respectively. IDA has a relatively strong binding affinity with His-tag proteins and has a higher loading capacity but lower specificity, while NTA has the opposite characteristics. Nickel and Cobalt ions have different properties at binding capacity of target proteins and non-specific adsorption. Nickel magnetic beads have a higher protein loading capacity but slightly lower purity (90%), while Cobalt magnetic beads have a slightly lower protein loading capacity but higher purity (95%). Users can select different types of His-tag protein purification beads based on different purposes.

TargetMol His-tag Protein Purification Magrose Beads IDA-Cobalt is a new type of superparamagnetic functional material designed for efficient and rapid purification of His-tag proteins. It can directly extract high-purity target proteins from biological samples in one step using magnetic separation method, which greatly simplifies the purification process and improves the efficiency. The method is suitable for scientific research and industrial areas to facilitate the purification of his-tag protein. The His-tag protein purification beads, based on magnetic agarose microspheres, activate and couple iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA). Then, they chelate Nickel and Cobalt ions respectively. IDA has a relatively strong binding affinity with His-tag proteins and has a higher loading capacity but lower specificity, while NTA has the opposite characteristics. Nickel and Cobalt ions have different properties at binding capacity of target proteins and non-specific adsorption. Nickel magnetic beads have a higher protein loading capacity but slightly lower purity (90%), while Cobalt magnetic beads have a slightly lower protein loading capacity but higher purity (95%). Users can select different types of His-tag protein purification beads based on different purposes.

TargetMol His-tag Protein Purification Magrose Beads IDA-Nickel is a new type of superparamagnetic functional material designed for efficient and rapid purification of His-tag proteins. It can directly extract high-purity target proteins from biological samples in one step using magnetic separation method, which greatly simplifies the purification process and improves the efficiency. The method is suitable for scientific research and industrial areas to facilitate the purification of his-tag protein. The His-tag protein purification beads, based on magnetic agarose microspheres, activate and couple iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA). Then, they chelate Nickel and Cobalt ions respectively. IDA has a relatively strong binding affinity with His-tag proteins and has a higher loading capacity but lower specificity, while NTA has the opposite characteristics. Nickel and Cobalt ions have different properties at binding capacity of target proteins and non-specific adsorption. Nickel magnetic beads have a higher protein loading capacity but slightly lower purity (90%), while Cobalt magnetic beads have a slightly lower protein loading capacity but higher purity (95%). Users can select different types of His-tag protein purification beads based on different purposes.