Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IHC, IF, FCM

Reactivity: Human, Mouse, Rabbit

IgG1

1N545

Human, Mouse, Rabbit

1. Western Blot
-Positive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissue
-All lanes: CD14 antibody at 1:2500
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 41 kDa
-Observed band size: 55 kDa
2. Western Blot
-Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
-All lanes: CD14 antibody at 1:1800
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 41 kDa
-Observed band size: 55 kDa
3. Immunofluorescence staining of A549 cells with TMAH-00166 at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
4. Immunofluorescence staining of Hela cells with TMAH-00166 at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
5. Immunofluorescence staining of HepG2 cells with TMAH-00166 at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
6. Immunofluorescence staining of RAW264.7 cells with TMAH-00166 at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
7. Immunofluorescence staining of SY5Y cells with TMAH-00166 at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
8. Overlay histogram showing Jurkat cells stained with TMAH-00166 (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
9. Overlay histogram showing RAW264.7 cells stained with TMAH-00166 (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, IF, FCM

Monoclonal

Unconjugated

Recombinant Protein: Human Monocyte differentiation antigen CD14 Protein (20-345AA)

Human

929

Immunology