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Anti-USP14 Antibody (2P900)
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Catalog No. TMAH-01236
Alias USP 14, Ubiquitin-specific-processing protease 14, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC 3.4.19.12, Deubiquitinating enzyme 14
Anti-USP14 Antibody (2P900) is an antibody targeting USP14. Anti-USP14 Antibody (2P900) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.
Anti-USP14 Antibody (2P900)
Anti-USP14 Antibody (2P900)
😃Good
Catalog No. TMAH-01236Alias USP 14, Ubiquitin-specific-processing protease 14, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC 3.4.19.12, Deubiquitinating enzyme 14
Anti-USP14 Antibody (2P900) is an antibody targeting USP14. Anti-USP14 Antibody (2P900) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
| Alias | USP 14, Ubiquitin-specific-processing protease 14, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC 3.4.19.12, Deubiquitinating enzyme 14 |
| Ig Type | Rabbit IgG |
| Clone | 2P900 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate -All lanes: USP14 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 57, 53, 55 kDa -Observed band size: 55-72 kDa 2. Immunofluorescence staining of PC-3 cell with TMAH-01236 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 517-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:2000; IF:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human USP14 |
| Antigen Species | Human |
| Gene ID | 9097 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cell biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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