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Anti-CD9 Antibody (6A19)
😃Good
Catalog No. TMAY-01496
Alias TSPAN-29, TSPAN29, MRP-1, MIC3, DRAP-27, CD9 molecule, BTCC-1
Anti-CD9 Antibody (6A19) is a Mouse antibody targeting CD9. Anti-CD9 Antibody (6A19) can be used in ELISA,FCM,ICC/IF.
Anti-CD9 Antibody (6A19)
Anti-CD9 Antibody (6A19)
😃Good
Catalog No. TMAY-01496Alias TSPAN-29, TSPAN29, MRP-1, MIC3, DRAP-27, CD9 molecule, BTCC-1
Anti-CD9 Antibody (6A19) is a Mouse antibody targeting CD9. Anti-CD9 Antibody (6A19) can be used in ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $69 | 7-10 days | |
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, FCM, ICC/IF Reactivity: Human |
| Alias | TSPAN-29, TSPAN29, MRP-1, MIC3, DRAP-27, CD9 molecule, BTCC-1 |
| Ig Type | Mouse IgG1 |
| Clone | 6A19 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human CD9 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD9 monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Flow cytometric analysis of Human CD9 expression on HeLa cells. Cells were stained with purified anti-Human CD9, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:50-1:1000; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD9 (rh CD9; TMPY-01806; NP_001760.1; Ser 112-Ile 195). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD9 is a member of the transmembrane 4 superfamily, which is also known as the tetraspanin family. CD9 is a cell surface glycoprotein with 4 hydrophobic domains that are described as complex with integrins and other transmembrane 4 superfamily members. It is found expressed on the surface of the exosomes. The protein takes part in cellular signal transduction events and thus play a role in the regulation of cell development and activation, growth and motility. Besides, CD9 seems to be a key role in the egg-sperm fusion during the mammalian fertilization processes. CD9 is found on the membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli. |
| Conjucates | Unconjugated |
| Others Formats | APC/PerCP/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Protein: Human CD9 protein (TMPY-01806) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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