1. Measured by its binding ability in a functional ELISA. Immobilized EPOR (hFc Tag) at 2μg/mL (100μL/well) can bind Cynomolgus Erythropoietin-His , the EC50 of Cynomolgus Erythropoietin-His is 4-20 ng/mL. 2. Measured by its ability to inhibit Epo-­dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1-4 ng/ml in the presence of 0.1 U/mL Recombinant Human EPO.

EPOR Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 51.0 kDa and the accession number is P19235-1.

Human

HEK293 Cells

erythropoietin receptor,EPO-R

A DNA sequence encoding the extracellular domain (Met-Pro 250) of human erythropoietin receptor (NP_000112.1) precursor was expressed with the C-terminal fused Fc region of human IgG1. Predicted N terminal: Ala 25

> 90 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.