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ANGPT2/Angiopoietin-2 Protein, Human (His & Avi), Biotinylated
(Synonyms: Tie2-ligand, ANGPT2, angiopoitin 2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2, angiopoietin 2, ANG-2, ANG2, AGPT2) Copy Product InfoSynonyms: Tie2-ligand, ANGPT2, angiopoitin 2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2, angiopoietin 2, ANG-2, ANG2, AGPT2
Catalog No. TMPK-00304 Copy Product Info
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 100 μg | $814 | 7-10 days | 7-10 days | |
| 500 μg | $3,250 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Immobilized Anti-ANGPT2 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ANGPT2, His Tag with the EC50 of 0.12μg/ml determined by ELISA.
2. Human TIE2, His Tag immobilized on CM5 Chip can bind Biotinylated Human ANGPT2, His Tag with an affinity constant of 2.93 μM as determined in SPR assay. |
| Description | Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. |
| Species | Human |
| Expression System | HEK293 |
| Tag | N-His-Avi |
| Accession Number | O15123-1 |
| Construction | Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Lys275-Phe496.[Accession |O15123-1] |
| Protein Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability & Storage | -20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Synonyms | Tie2-ligand, ANGPT2, angiopoitin 2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2, angiopoietin 2, ANG-2, ANG2, AGPT2 |
| Research Background |
| Molecular Weight | 28.4 kDa (predicted). Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Related Tags: ANGPT2/Angiopoietin-2 Protein, Human (His & Avi), Biotinylated chemical structure | ANGPT2/Angiopoietin-2 Protein, Human (His & Avi), Biotinylated molecular weight
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