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Anti-Phospho-HSPB1 (Ser82) Antibody (8A434) is an antibody targeting Phospho-HSPB1 (Ser82). Anti-Phospho-HSPB1 (Ser82) Antibody (8A434) can be used in ELISA, WB, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
Alias | SRP27, p-HSPB1 (Ser82), p-HSPB1 (S82), Phospho-HSPB1 (S82), HSPB1 (p-Ser82), HSPB1 (p-S82), HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F |
Ig Type | Rabbit IgG |
Clone | 8A434 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in Hela whole cell lysate, HepG2 whole cell lysate(treated with Calyculin A or EGF) -All lanes Phospho-HSPB1 antibody at 0.73μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 27 KDa -Observed band size: 27 KDa 2. IHC image of TMAH-00911 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Application | ELISA, WB, IHC |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human Phospho-HSPB1 (S82) |
Antigen Species | Human |
Gene ID | 3315 |
Uniprot ID | |
Biology Area | Signal Transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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