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Anti-Phospho-Serine/Threonine Polyclonal Antibody is a Rabbit antibody targeting Phospho-Serine/Threonine. Anti-Phospho-Serine/Threonine Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Phospho-Serine/Threonine Polyclonal Antibody is a Rabbit antibody targeting Phospho-Serine/Threonine. Anti-Phospho-Serine/Threonine Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,ELISA. |
Alias | p-SER/p-Thr, pSER/pThr, p-S/p-T, pS/pT, phosphoserine/threonine, phospho-Ser/phospho-Thr, pan p-Sp-T, pan p-SER/p-Thr, pan pSER/pThr, pan p-S/p-T, pan pS/pT, pan phospho-Ser/phospho-Thr |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphoserine,threonine) Polyclonal Antibody, Unconjugated (TMAB-01491) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphoserine,threonine) Polyclonal Antibody, Unconjugated (TMAB-01491) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphoserine,threonine) Polyclonal Antibody, Unconjugated (TMAB-01491) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Protein phosphorylation provides a signalling system that can be thought of as a kind of protein on/off switch for many cellular signalling pathways. Phosphorylation is observed on serine, threonine, tyrosine and histidine residues. Cellular networks underlying phosphorylation can be very complex and often occurs on multiple distinct sites on a given protein. Phospho-specific antibodies are becoming critical reagents both for basic research and for clinical diagnosis. |
Immunogen | KLH conjugated synthesised phosphopeptide contain Phosphoserine and Phosphothreonine |
Biology Area | Amino acid metabolism,Phosphatases,Amino Acids,Tyrosine Phosphatases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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