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Anti-Phospho-Histone H3 (Ser28) Antibody (4M267) is a Mouse antibody targeting Phospho-Histone H3 (Ser28). Anti-Phospho-Histone H3 (Ser28) Antibody (4M267) can be used in ELISA,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $209 | 7-10 days | 7-10 days | |
| 100 μL | $349 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-Histone H3 (Ser28) Antibody (4M267) is a Mouse antibody targeting Phospho-Histone H3 (Ser28). Anti-Phospho-Histone H3 (Ser28) Antibody (4M267) can be used in ELISA,FCM. |
| Synonyms | Phospho-Histone H3(S28), p-Histone H3(S28), p-Histone H3 (Ser28), Histone H3.1, Histone H3(p-S28), Histone H3 (p-Ser28), Histone H3, histone cluster 1, H3a, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J |
| Ig Type | IgG1 |
| Clone | 4M267 |
| Reactivity | Human |
| Verified Activity | 1. Overlay Peak curve showing Hela cells stained with TMAH-00905 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. 2. Overlay Peak curve showing HepG2 cells stained with TMAH-00905 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nucleus. Chromosome. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Purity | >95% |
| Research Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Conjucates | Unconjugated |
| Immunogen | KS(Phospho)APATGGVKKPHRC |
| Antigen Species | Human |
| Gene Name | HIST1H3A |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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