Anti-Azurocidin/HBP Antibody (9H146) is a Mouse antibody targeting Azurocidin/HBP. Anti-Azurocidin/HBP Antibody (9H146) can be used in WB,ELISA,IHC-P,IHC-Fr,IF.

IgG1

9H146

Human

1. Measured by its binding ability in a indirect ELISA. Immobilized Human Azurocidin, His Tag at 2 μg/mL (100 μL/well) can bind Anti-HumanAzurocidin monoclonal antibody, the minimum detection concentration is 4 ng/mL
2. Sample:
Lane 1: Recombinant Human Azurocidin Protein at 100 ng
Primary: Mouse Anti-Azurocidin Monoclonal Antibody at 1/1000 dilution (Cat. TMAB-02989)
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28.6kD
Observed band size: 38-42 kD
3. Paraformaldehyde-fixed, paraffin embedded Human Acute choriitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (TMAB-02989) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (TMAB-02989) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (TMAB-02989) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Azurocidin/HBP Monoclonal Antibody, Unconjugated (TMAB-02989) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.

WB: 1:500-2000; ELISA: 1:5000-10000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: Human Azurocidin Protein

Human

AZU1

Azurocidin

This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.