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Siglec-3/CD33 Protein, Human, Recombinant

(Synonyms: Siglec-3, SIGLEC3, p67, CD33 molecule) Copy Product Info

Synonyms: Siglec-3, SIGLEC3, p67, CD33 molecule

Catalog No. TMPY-04805 Copy Product Info
Siglec-3/CD33 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 27.4 kDa and the accession number is AAH28152.1.

Batch Information

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Purity:SDS-PAGE: 97.1%; SEC-HPLC: 99.2%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Recombinant Human CD33/Siglec-3 Protein (Cat#TMPY-04805) at 2 μg/ml (100 μl/well) can bind Anti-CD33 Antibody (Lintuzumab), Human IgG1, the EC50 is 3-10 ng/mL.
Description
Siglec-3/CD33 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 27.4 kDa and the accession number is AAH28152.1.
Species
Human
Expression System
HEK293 Cells
TagTag Free
Accession NumberAAH28152.1
ConstructionA DNA sequence encoding the human CD33 (AAH28152.1) (Met1-His259) was expressed with five amino acids (DDDDK) at the C-terminus. Predicted N terminal: Asp 18
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Siglec-3/CD33 Protein, Human, RecombinantSiglec-3/CD33 Protein, Human, Recombinant
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsSiglec-3, SIGLEC3, p67, CD33 molecule
Research Background
Chemical Properties
Molecular Weight27.4 kDa (predicted)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: Siglec-3/CD33 Protein, Human, Recombinant chemical structure | Siglec-3/CD33 Protein, Human, Recombinant molecular weight