IGFBP-4 Protein, Human, Recombinant (His)

Name: IGFBP-4 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-00836
Price: 55 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: insulin-like growth factor binding protein 4, IGFBP-4, IBP4, HT29-IGFBP, BP-4) Copy Product Info

Synonyms: insulin-like growth factor binding protein 4, IGFBP-4, IBP4, HT29-IGFBP, BP-4

Catalog No. TMPY-00836 Copy Product Info

IGFBP-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.4 kDa and the accession number is A0A024R1U8.

Pack Size	Price	USA Stock	Global Stock
5 µg	$55	-	In Stock
10 µg	$86	-	In Stock
20 µg	$139	-	In Stock
50 µg	$269	-	In Stock
100 µg	$451	-	In Stock
200 µg	$761	7-10 days	7-10 days
500 µg	$1,510	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:94.8%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Measured by its ability to bind human IGF1 in functional ELISA. 2. Measured by its ability to bind human IGF2 in functional ELISA. 3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 0.04-0.4 μg/mL in the presence of 14 ng/mL human IGFII.
Description	IGFBP-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.4 kDa and the accession number is A0A024R1U8.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P22692
Construction	A DNA sequence encoding the human IGFBP4 (NP_001543.2) precursor (Met 1-Glu 258) with a C-terminal polyhistidine tag was expressed. Predicted N terminal: Asp 22
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	insulin-like growth factor binding protein 4, IGFBP-4, IBP4, HT29-IGFBP, BP-4
Research Background	Insulin-like growth factor-binding protein 4 (IGFBP-4) is a 24-kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF or act in an IGF-independent manner in the prostate. IGF-binding protein-4 (IGFBP-4) inhibits IGF-I action in vitro and is the most abundant IGFBP in the rodent arterial wall. Expression of IGFBP-4 mRNA in nontransgenic littermates was maximal in the liver and kidney. IGFBP-4 is a functional antagonist of IGF-I action on SMC. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence, and IGF affinity.

Chemical Properties

Molecular Weight

27.4 kDa (predicted); 32 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.