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Anti-MESDC2 Antibody (5E452) is a Rabbit antibody targeting MESDC2. Anti-MESDC2 Antibody (5E452) can be used in WB,ELISA,ICC/IF,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $269 | 7-10 days |
Description | Anti-MESDC2 Antibody (5E452) is a Rabbit antibody targeting MESDC2. Anti-MESDC2 Antibody (5E452) can be used in WB,ELISA,ICC/IF,IP. |
Alias | mesoderm development candidate 2, MESD, BOCA |
Ig Type | Monoclonal Rabbit IgG |
Clone | 5E452 |
Reactivity | Human |
Verified Activity | 1. Anti-MESDC2 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HEK293 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:26 kDa. 2. MESDC2 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -Lane C:0.5 mg NIH-3T3 Whole Cell Lysate. -2 µL anti-MESDC2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-MESDC2 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 26 kDa. -Observed band size: 26 kDa. 3. Immunofluorescence staining of Mesdc2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human Mesdc2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm. |
Application | WB,ELISA,ICC/IF,IP |
Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human MESDC2 (rh MESDC2; TMPY-01451; NP_055969.1; Ala 34-Lys 230). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | MESD (Mesoderm Development LRP Chaperone) is a Protein Coding gene. LDLR chaperone MESD, also known as Mesoderm development protein, Mesoderm development candidate 2, Renal carcinoma antigen NY-REN-61, and MESDC2, is a member of the MESD family. It is widely expressed in the thyroid, placenta, and other tissues. MESDC2 is a chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). The LDLR maturation activity resides in the N- and C-terminal unstructured regions. MESDC2 acts as a modulator of the Wnt pathway since some LDLRs are coreceptors for the canonical Wnt pathway. MESDC2 is essential for the specification of embryonic polarity and mesoderm induction. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human MESDC2 protein (TMPY-01451) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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