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Anti-MESDC2 Antibody (5E452)

😃Good
Catalog No. TMAY-01374
Alias mesoderm development candidate 2, MESD, BOCA

Anti-MESDC2 Antibody (5E452) is a Rabbit antibody targeting MESDC2. Anti-MESDC2 Antibody (5E452) can be used in WB,ELISA,ICC/IF,IP.

Anti-MESDC2 Antibody (5E452)

Anti-MESDC2 Antibody (5E452)

😃Good
Catalog No. TMAY-01374Alias mesoderm development candidate 2, MESD, BOCA
Anti-MESDC2 Antibody (5E452) is a Rabbit antibody targeting MESDC2. Anti-MESDC2 Antibody (5E452) can be used in WB,ELISA,ICC/IF,IP.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MESDC2 Antibody (5E452) is a Rabbit antibody targeting MESDC2. Anti-MESDC2 Antibody (5E452) can be used in WB,ELISA,ICC/IF,IP.
Aliasmesoderm development candidate 2, MESD, BOCA
Ig Type
Monoclonal Rabbit IgG
Clone
5E452
Reactivity
Human
Verified Activity
1. Anti-MESDC2 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HEK293 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:26 kDa.

-Observed band size:26 kDa.

2. MESDC2 was immunoprecipitated using:

-Lane A:0.5 mg 293T Whole Cell Lysate.

-Lane B:0.5 mg Hela Whole Cell Lysate.

-Lane C:0.5 mg NIH-3T3 Whole Cell Lysate.

-2 µL anti-MESDC2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-MESDC2 rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 26 kDa.

-Observed band size: 26 kDa.

3. Immunofluorescence staining of Mesdc2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human Mesdc2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.
Application
WB,ELISA,ICC/IF,IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human MESDC2 (rh MESDC2; TMPY-01451; NP_055969.1; Ala 34-Lys 230).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundMESD (Mesoderm Development LRP Chaperone) is a Protein Coding gene. LDLR chaperone MESD, also known as Mesoderm development protein, Mesoderm development candidate 2, Renal carcinoma antigen NY-REN-61, and MESDC2, is a member of the MESD family. It is widely expressed in the thyroid, placenta, and other tissues. MESDC2 is a chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). The LDLR maturation activity resides in the N- and C-terminal unstructured regions. MESDC2 acts as a modulator of the Wnt pathway since some LDLRs are coreceptors for the canonical Wnt pathway. MESDC2 is essential for the specification of embryonic polarity and mesoderm induction.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human MESDC2 protein (TMPY-01451)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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