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Anti-Histone H2B type 1-K Antibody (6I202)

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Catalog No. TMAH-00552

Anti-Histone H2B type 1-K Antibody (6I202) is an antibody targeting Histone H2B type 1-K. Anti-Histone H2B type 1-K Antibody (6I202) can be used in ELISA, WB, IHC.

Anti-Histone H2B type 1-K Antibody (6I202)

Anti-Histone H2B type 1-K Antibody (6I202)

😃Good
Catalog No. TMAH-00552
Anti-Histone H2B type 1-K Antibody (6I202) is an antibody targeting Histone H2B type 1-K. Anti-Histone H2B type 1-K Antibody (6I202) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$207 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human
Ig Type
Rabbit IgG
Clone
6I202
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue
-All lanes: Histone H2B type 1-K antibody at 0.16μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 14 KDa
-Observed band size: 14 KDa
2. IHC image of TMAH-00552 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:5000-1:10000; IHC:1:50-1:500.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus. Chromosome.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide
Antigen Species
Human
Gene ID
85236
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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