Anti-GFAP Polyclonal Antibody 2 is a Rabbit antibody targeting GFAP. Anti-GFAP Polyclonal Antibody 2 can be used in WB,ICC/IF,ELISA.

IgG

Human

1. Measured by its binding ability in a indirect ELISA. Immobilized Human GFAP protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human GFAP Antibody, the EC50 is 540.1 ng/mL.
2. 25 μg total protein per lane of various lysates (see on figure) probed with GFAP polyclonal antibody, unconjugated (TMAB-06443) at 1: 20000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. 4% Paraformaldehyde-fixed U87-MG (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (GFAP) polyclonal Antibody, unconjugated (TMAB-06443) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:5000-20000; ICC/IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: human GFAP protein

Human

GFAP

Glial fibrillary acidic protein

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.