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Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (hFc)
(Synonyms: Human cytωlovirus (HCMV) Glycoprotein B/gB) Copy Product InfoSynonyms: Human cytωlovirus (HCMV) Glycoprotein B/gB
Catalog No. TMPY-00810 Copy Product Info
Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (hFc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 118 kDa and the accession number is P13201.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $95 | - | In Stock | |
| 10 µg | $155 | - | In Stock | |
| 20 µg | $257 | - | In Stock | |
| 50 µg | $497 | 7-10 days | 7-10 days | |
| 100 µg | $987 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 80 % as determined by SDS-PAGE
Appearance:Lyophilized powder
Color:White
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| Bioactivity | Measured by its ability to bind biotinylated Human CD209-Fc in functional ELISA. |
| Description | Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (hFc) is expressed in HEK293 mammalian cells with Fc tag. The predicted molecular weight is 118 kDa and the accession number is P13201. |
| Species | CMV |
| Expression System | HEK293 Cells |
| Tag | C-hFc |
| Accession Number | P13201 |
| Construction | A DNA sequence encoding the extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907) of human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') precursor was fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Asn 25 |
| Protein Purity | > 80 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Gly, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Synonyms | Human cytωlovirus (HCMV) Glycoprotein B/gB |
| Research Background | Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium. |
| Molecular Weight | 118 kDa (predicted); 160-170 kDa (reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (hFc) chemical structure | Human cytomegalovirus (HCMV) Glycoprotein B/gB Protein (hFc) molecular weight
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