SARS-CoV-2 Spike S1+S2 Protein (L18F & D80A & D215G & LAL242-244 deletion & R246I & K417N & E484K & N501Y & D614G & A701V, His), Biotinylated

Name: SARS-CoV-2 Spike S1+S2 Protein (L18F & D80A & D215G & LAL242-244 deletion & R246I & K417N & E484K & N501Y & D614G & A701V, His), Biotinylated
Brand: TargetMol
SKU: TMPY-06171
Rating: 4.5 (1 reviews)

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Catalog No. TMPY-06171 Copy Product Info

SARS-CoV-2 Spike S1+S2 Protein (L18F & D80A & D215G & LAL242-244 deletion & R246I & K417N & E484K & N501Y & D614G & A701V, His), Biotinylated is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 134 kDa and the accession number is A0A6G7K2L4.

Pack Size	Price	USA Stock	Global Stock
5 μg	$250	7-10 days	7-10 days
10 μg	$419	7-10 days	7-10 days
20 μg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1+S2 (L18F, D80A, D215G, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag), Biotinylated, the EC50 of SARS-CoV-2 Spike S1+S2 (L18F, D80A, D215G, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, A701V) Protein (ECD, His Tag), Biotinylated is 50-350 ng/mL.
Description	SARS-CoV-2 Spike S1+S2 Protein (L18F & D80A & D215G & LAL242-244 deletion & R246I & K417N & E484K & N501Y & D614G & A701V, His), Biotinylated is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 134 kDa and the accession number is A0A6G7K2L4.
Species	SARS-CoV-2
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	A0A6G7K2L4
Construction	A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S1+S2 (YP_009724390.1, with mutations L18F, D80A, D215G, LAL242-244 deletion, R246I, K417N, E484K, N501Y, D614G, A701V) (Met1-Pro1213) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. The mutations were identified in the SARS-CoV-2 variant (known as 20C/501Y.V2 or B.1.351 lineage) which emerged in South Africa. Predicted N terminal: Val 16
Protein Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (contains 300 mM NaCl), pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background

Chemical Properties

Molecular Weight

134 kDa (predicted)

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: SARS-CoV-2 Spike S1+S2 Protein (L18F & D80A & D215G & LAL242-244 deletion & R246I & K417N & E484K & N501Y & D614G & A701V, His), Biotinylated chemical structure | SARS-CoV-2 Spike S1+S2 Protein (L18F & D80A & D215G & LAL242-244 deletion & R246I & K417N & E484K & N501Y & D614G & A701V, His), Biotinylated molecular weight