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Anti-Nectin-2 Antibody (1C307)
🥰Excellent
Catalog No. TMAY-03229
Anti-Nectin-2 Antibody (1C307) is a Rabbit antibody targeting Nectin-2. Anti-Nectin-2 Antibody (1C307) can be used in WB,ELISA,IP.
Anti-Nectin-2 Antibody (1C307)
Anti-Nectin-2 Antibody (1C307)
🥰Excellent
Catalog No. TMAY-03229
Anti-Nectin-2 Antibody (1C307) is a Rabbit antibody targeting Nectin-2. Anti-Nectin-2 Antibody (1C307) can be used in WB,ELISA,IP.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Nectin-2 Antibody (1C307) is a Rabbit antibody targeting Nectin-2. Anti-Nectin-2 Antibody (1C307) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 1C307 |
| Reactivity | Mouse |
| Verified Activity | 1. Anti-mPVRL2 rabbit monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:58 kDa. -Observed band size:58 kDa(We are unsure as to the identity of these extra bands.) 2. Mouse PVRL2 was immunoprecipitated using: -Lane A:0.5 mg Raji Whole Cell Lysate -0.5 µL anti-Mouse PVRL2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse PVRL2 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 58 kDa. -Observed band size: 58 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000; IP: 0.2-1 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PVRL2 extracellular domain (rM PVRL2; TMPY-01150; NP_033016.3; Met 1-Gly 351). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Mouse PVRL2 protein (TMPY-01150) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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