Immobilized Mouse NKG2D, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 0.39 μg/ml determined by ELISA.

NKG2D/CD314 Protein, Mouse, Recombinant (aa 90-232, His) is expressed in HEK293 Cells. The accession number is O54709-1.

Mouse

HEK293 Cells

NKG2-D,Nkg2d,Klrk1,D6H12S2489E

Phe90-Val232

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.