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Anti-MFN2 Polyclonal Antibody is a Rabbit antibody targeting MFN2. Anti-MFN2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days |
Description | Anti-MFN2 Polyclonal Antibody is a Rabbit antibody targeting MFN2. Anti-MFN2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse) |
Verified Activity | 1. Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Mitofusin-2 Polyclonal Antibody, Unconjugated (TMAB-01132) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C. 2. Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Mitofusin 2 Polyclonal Antibody, Unconjugated (TMAB-01132) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Mitofusin 2) Polyclonal Antibody, Unconjugated (TMAB-01132) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Sample: Lane 1: Mouse Muscle tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Rat Muscle tissue lysates Lane 4: Rat Cerebrum tissue lysates Primary: Anti-Mitofusin 2 (TMAB-01132) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83 kDa Observed band size: 77 kDa 5. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-Mitofusin 2 (TMAB-01132) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83 kDa Observed band size: 68 kDa |
Application | WB,IHC-P,IHC-Fr,IF,ELISA |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Mitochondrion outer membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]. |
Immunogen | KLH conjugated synthetic peptide: human Mitofusin 2 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Mitochondria,Mitochondrial,Neurodegenerative disease,Mitophagy fission and fusion,Mitochondrial markers,Apoptosis,Mitochondrial,Mitochondrial,Metabolism |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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