Antibody Type: Recombinant Monoclonal

Application: ELISA, IHC, FC

Reactivity: Human

Mouse IgG2a

9K489

Human

1. IHC image of TMAH-00999 diluted at 1:130 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
2. Overlay Peak curve showing Hela cells stained with TMAH-00999 (red line) at 1:200. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,020 events was performed.

ELISA, IHC, FC

IHC:1:20-1:200; FC:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human PRDX2 Protein

Human

7001

Neuroscience, Cancer, Cell biology, Metabolism