Immobilized Human DKK1, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 1.7 μg/ml determined by ELISA.

DKK1 Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is O94907.

Human

HEK293 Cells

SK,DKK-1,dickkopf WNT signaling pathway inhibitor 1

Thr32-His266

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.