Immobilized Human CD74 at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 8.9 ng/ml determined by ELISA.

CD74 Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is NP_004346.1.

Human

HEK293 Cells

II,Ia-γ,Ia-GAMMA,HLADG,DHLAG,CD74 molecule, major histocompatibility complex, class II invariant chain

Gln73-Met232

> 95% as determined by Tris-Bis PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.