Antibody Type: Recombinant Mouse Monoclonal

Application: ELISA, WB, IHC, IF, FCM

Reactivity: Human, Mouse

Mouse IgG2a

4H35

Human, Mouse

1. Western Blot
-Positive WB detected in: 293 whole cell lysate
-All lanes: CALR antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 49 kDa
-Observed band size: 49 kDa
2. IHC image of TMAH-00134 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
3. Immunofluorescence staining of HepG2 cell with TMAH-00134 at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
4. Overlay Peak curve showing JK cells stained with TMAH-00134 (red line) at 1:300. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,018 events was performed.

ELISA, WB, IHC, IF, FCM

WB:1:1000-1:5000; IHC:1:20-1:200; IF:1:20-1:200; FCM:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human CALR Protein

Human

811

Tags & Cell Markers