IL-10 Protein, Human, Recombinant

Name: IL-10 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-03547
Rating: 4.5 (1 reviews)

(Synonyms: TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF) Copy Product Info

Synonyms: TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF

Catalog No. TMPY-03547 Copy Product Info

IL-10 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.6 kDa and the accession number is P22301.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$108	7-10 days	7-10 days
20 µg	$177	In Stock	In Stock
50 µg	$298	7-10 days	7-10 days
100 µg	$447	7-10 days	7-10 days
200 µg	$791	7-10 days	7-10 days
500 µg	Preferential	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:> 95 % as determined by SDS-PAGE.

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Immobilized human IL10 at 10 μg/mL (100 μl/well) can bind Cynomolgus IL10RA-Fc , The EC50 of Cynomolgus IL10RA-Fc is 0.24-0.56 μg/mL. 2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 0.5-2.5 ng/mL.
Description	IL-10 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.6 kDa and the accession number is P22301.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P22301
Construction	A DNA sequence encoding human IL10 (NP_000563.1) (Ser 19-Asn 178) was expressed. Predicted N terminal: Ser 19
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 20 mM NaCl, pH 6. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF
Research Background	IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of some cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen-presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

18.6 kDa (predicted); 18 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.