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Anti-Fibronectin Antibody (5U855)
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Catalog No. TMAH-00447
Alias FN1, Fibronectin
Anti-Fibronectin Antibody (5U855) is an antibody targeting Fibronectin. Anti-Fibronectin Antibody (5U855) can be used in ELISA, FCM.
Anti-Fibronectin Antibody (5U855)
Anti-Fibronectin Antibody (5U855)
😃Good
Catalog No. TMAH-00447Alias FN1, Fibronectin
Anti-Fibronectin Antibody (5U855) is an antibody targeting Fibronectin. Anti-Fibronectin Antibody (5U855) can be used in ELISA, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Mouse Monoclonal Application: ELISA, FCM Reactivity: Human |
| Alias | FN1, Fibronectin |
| Ig Type | Mouse IgG2a |
| Clone | 5U855 |
| Reactivity | Human |
| Verified Activity | Overlay Peak curve showing 3T3 cells stained with TMAH-00447 (red line) at 1:50.The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
| Application | ELISA, FCM |
| Recommended Dose | FCM:1:20-1:500. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human FN1 Protein |
| Antigen Species | Human |
| Gene ID | 2335 |
| Uniprot ID | |
| Biology Area | Cancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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