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CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His)
(Synonyms: Fc receptor, IgG, low affinity III, CD16) Copy Product InfoSynonyms: Fc receptor, IgG, low affinity III, CD16
Catalog No. TMPY-01834 Copy Product Info
Purity: 99.4%
CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.6 kDa and the accession number is P08508-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $48 | - | In Stock | |
| 10 µg | $75 | - | In Stock | |
| 20 µg | $118 | - | In Stock | |
| 50 µg | $229 | 7-10 days | 7-10 days | |
| 100 µg | $386 | - | In Stock | |
| 200 µg | $682 | 7-10 days | 7-10 days | |
| 500 µg | $1,430 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:99.4%
Appearance:Lyophilized powder
Color:White
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Product Information
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Measured by its ability to bind Human IgG1 , Biotinylated in a functional ELISA.
2.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a on ProA Biosensor, can bind Recombinant Mouse CD16/FCGR3 Protein, His Tag with an affinity constant of 322nM as determined in BLI assay(QC tested). |
| Description | CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.6 kDa and the accession number is P08508-1. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P08508-1 |
| Construction | A DNA sequence encoding the mouse FCGR3 (P08508-1) extracellular domain (Met 1-Thr 215) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 31 |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.  , |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Synonyms | Fc receptor, IgG, low affinity III, CD16 |
| Research Background | Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes. |
| Molecular Weight | 22.6 kDa (predicted); 35-44 kDa (reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His) chemical structure | CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His) molecular weight
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