CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His)

Catalog No. TMPY-01834 Copy Product Info

CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.6 kDa and the accession number is P08508-1.

CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His)

Catalog No. TMPY-01834

Copy Product Info

CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.6 kDa and the accession number is P08508-1.

Pack Size	Price	USA Stock	Global Stock
5 μg	$48	-	In Stock
10 μg	$75	-	In Stock
20 μg	$118	-	In Stock
50 μg	$229	7-10 days	7-10 days
100 μg	$386	-	In Stock
200 μg	$682	7-10 days	7-10 days
500 μg	$1,430	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	1.Measured by its ability to bind Human IgG1 , Biotinylated in a functional ELISA. 2.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a on ProA Biosensor, can bind Recombinant Mouse CD16/FCGR3 Protein, His Tag with an affinity constant of 322nM as determined in BLI assay(QC tested).
Description	CD16/FCGR3 Protein, Mouse, Recombinant (aa 32-215, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.6 kDa and the accession number is P08508-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P08508-1
Synonyms	Fc receptor, IgG, low affinity III,CD16
Construction	A DNA sequence encoding the mouse FCGR3 (P08508-1) extracellular domain (Met 1-Thr 215) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 31
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Molecular Weight	22.6 kDa (predicted); 35-44 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.