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Anti-BUB1B Antibody (4Y527)
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Catalog No. TMAH-00125
Alias Protein SSK1, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Mitotic checkpoint kinase MAD3L, MAD3/BUB1-related protein kinase, hBUBR1, EC 2.7.11.1, BUBR1 MAD3L SSK1, BUB 1B
Anti-BUB1B Antibody (4Y527) is an antibody targeting BUB1B. Anti-BUB1B Antibody (4Y527) can be used in ELISA, WB, FCM.
Anti-BUB1B Antibody (4Y527)
Anti-BUB1B Antibody (4Y527)
😃Good
Catalog No. TMAH-00125Alias Protein SSK1, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Mitotic checkpoint kinase MAD3L, MAD3/BUB1-related protein kinase, hBUBR1, EC 2.7.11.1, BUBR1 MAD3L SSK1, BUB 1B
Anti-BUB1B Antibody (4Y527) is an antibody targeting BUB1B. Anti-BUB1B Antibody (4Y527) can be used in ELISA, WB, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, FCM Reactivity: Human |
| Alias | Protein SSK1, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Mitotic checkpoint kinase MAD3L, MAD3/BUB1-related protein kinase, hBUBR1, EC 2.7.11.1, BUBR1 MAD3L SSK1, BUB 1B |
| Ig Type | Rabbit IgG |
| Clone | 4Y527 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate -All lanes: BubR1 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 120, 106, 122 kDa -Observed band size: 125 kDa 2. Overlay histogram showing Hela cells stained with TMAH-00125 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, FCM |
| Recommended Dose | WB:1:500-1:5000; FCM:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human BubR1 |
| Antigen Species | Human |
| Gene ID | 701 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer, Cell biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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