Anti-HMGCS2 Antibody (2I93) is an antibody targeting HMGCS2. Anti-HMGCS2 Antibody (2I93) can be used in ELISA, WB, IHC.

Rabbit IgG

2I93

Human

1. Western Blot
-Positive WB detected in: A549 whole cell lysate, Ntera-2 whole cell lysate
-All lanes: HMGCS2 antibody at 1:500
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 57, 53, 51 kDa
-Observed band size: 55 kDa
2. IHC image of TMAH-00561 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00561 diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

ELISA, WB, IHC

WB:1:500-1:2000; IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human HMGCS2

Human

3158

Cancer, Cardiovascular, Metabolism, Signal transduction