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Anti-HMGCS2 Antibody (2I93) is an antibody targeting HMGCS2. Anti-HMGCS2 Antibody (2I93) can be used in ELISA,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $209 | 7-10 days | 7-10 days | |
| 100 μL | $347 | 7-10 days | 7-10 days |
| Description | Anti-HMGCS2 Antibody (2I93) is an antibody targeting HMGCS2. Anti-HMGCS2 Antibody (2I93) can be used in ELISA,IHC,WB. |
| Synonyms | mitochondrial, Hydroxymethylglutaryl-CoA synthase, HMGCS 2, HMG-CoA synthase, EC 2.3.3.10, 3-hydroxy-3-methylglutaryl coenzyme A synthase |
| Ig Type | Rabbit IgG |
| Clone | 2I93 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: A549 whole cell lysate, Ntera-2 whole cell lysate -All lanes: HMGCS2 antibody at 1:500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 57, 53, 51 kDa -Observed band size: 55 kDa 2. IHC image of TMAH-00561 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00561 diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
| Application | |
| Recommended Dose | WB:1:500-1:2000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Mitochondrion. |
| Tissue Specificity | Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain.; [Isoform 3]: Highest expression detected in heart and skeleta |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Catalyzes the first irreversible step in ketogenesis, condensing acetyl-CoA to acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human HMGCS2 |
| Antigen Species | Human |
| Gene Name | HMGCS2 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cancer, Cardiovascular, Metabolism, Signal transduction |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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