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Anti-Phospho-SMAD2 (Ser255) Antibody (1S198)
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Catalog No. TMAH-00964
Alias SMAD2 (p-Ser255), SMAD2 (p-S255), p-SMAD2 (Ser255), p-SMAD2 (S255), Phospho-SMAD2 (S255)
Anti-Phospho-SMAD2 (Ser255) Antibody (1S198) is an antibody targeting Phospho-SMAD2 (Ser255). Anti-Phospho-SMAD2 (Ser255) Antibody (1S198) can be used in ELISA, IF.
Anti-Phospho-SMAD2 (Ser255) Antibody (1S198)
Anti-Phospho-SMAD2 (Ser255) Antibody (1S198)
😃Good
Catalog No. TMAH-00964Alias SMAD2 (p-Ser255), SMAD2 (p-S255), p-SMAD2 (Ser255), p-SMAD2 (S255), Phospho-SMAD2 (S255)
Anti-Phospho-SMAD2 (Ser255) Antibody (1S198) is an antibody targeting Phospho-SMAD2 (Ser255). Anti-Phospho-SMAD2 (Ser255) Antibody (1S198) can be used in ELISA, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, IF Reactivity: Human |
| Alias | SMAD2 (p-Ser255), SMAD2 (p-S255), p-SMAD2 (Ser255), p-SMAD2 (S255), Phospho-SMAD2 (S255) |
| Ig Type | Rabbit IgG |
| Clone | 1S198 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of A549 cells with TMAH-00964 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, IF |
| Recommended Dose | IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-SMAD2 (S255) |
| Antigen Species | Human |
| Gene ID | 4087 |
| Uniprot ID | |
| Biology Area | Signal Transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
SMAD2 (pSer255)SMAD2 (pS255)SMAD2 (phospho-Ser255)SMAD2 (phospho-S255)SMAD2SMAD family member 2 (SMAD 2;Smad2;hSMAD2)SMAD 2pSMAD2 (Ser255)pSMAD2 (S255)Mothers against DPP homolog 2Mothers against decapentaplegic homolog 2Mad-related protein 2 (hMAD-2)MADR2MADR 2MADH2MADH 2MAD homolog2MAD homolog 2JV18-1JV 18-1hMAD-2hMAD2
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