Antibody Type: Recombinant Monoclonal

Application: ELISA, IHC, IF, FC

Reactivity: Human

Rabbit IgG

6H879

Human

1. IHC image of TMAH-00430 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.
2. Immunofluorescence staining of Hela cell with TMAH-00430 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Overlay Peak curve showing MCF7 cells stained with TMAH-00430 (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was rabbit IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,026 events was performed.

ELISA, IHC, IF, FC

IHC:1:20-1:200; IF:1:20-1:200; FC:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human FGFR1 Protein

Human

2260

Epigenetics and Nuclear Signaling, Neuroscience, Cancer, Cardiovascular, Signal transduction