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Anti-CD146/MCAM Antibody (3X93)
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Catalog No. TMAY-00294
Alias MUC18, melanoma cell adhesion molecule, CD146
Anti-CD146/MCAM Antibody (3X93) is a Rabbit antibody targeting CD146/MCAM. Anti-CD146/MCAM Antibody (3X93) can be used in WB,ELISA,IHC-P.
Anti-CD146/MCAM Antibody (3X93)
Anti-CD146/MCAM Antibody (3X93)
😃Good
Catalog No. TMAY-00294Alias MUC18, melanoma cell adhesion molecule, CD146
Anti-CD146/MCAM Antibody (3X93) is a Rabbit antibody targeting CD146/MCAM. Anti-CD146/MCAM Antibody (3X93) can be used in WB,ELISA,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-CD146/MCAM Antibody (3X93) is a Rabbit antibody targeting CD146/MCAM. Anti-CD146/MCAM Antibody (3X93) can be used in WB,ELISA,IHC-P. |
| Alias | MUC18, melanoma cell adhesion molecule, CD146 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 3X93 |
| Reactivity | Human |
| Verified Activity | 1. Immunohistochemical staining of formalin fixed, paraffin-embedded human stomach showing membrane staining of endothelial cells (red arrow), epithelial cells (green arrow). The left panel: 1:200. The right panel: 1:1000. 2. Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200). 3. Immunohistochemical staining of formalin fixed, paraffin-embedded human kidney showing membrane staining of endothelial cells (red arrow) (1:200). 4. Anti-CD146 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:132 kDa. -Observed band size:140 kDa. 5. Anti-CD146 rabbit monoclonal antibody at 1:500 dilution. -Lane A: CD146 konckout Hela Whole Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 10 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:132 kDa. -Observed band size: kDa(Validation Experiment) |
| Application | WB,ELISA,IHC-P |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 / MCAM extracellular domain (rh CD146; TMPY-01034; NP_006491.2; Met 1-Gly 559) . |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region, and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumors, and thus has been suggested to play an important role in tumor progression and the development of metastasis in certain human carcinomas. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human CD146 protein (TMPY-01034) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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