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Anti-LRPAP1 Antibody (6M356)
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Catalog No. TMAY-01642
Alias RAP, low density lipoprotein receptor-related protein associated protein 1, HBP44, C77774, AU042172, AI790446, AA617339
Anti-LRPAP1 Antibody (6M356) is a Rabbit antibody targeting LRPAP1. Anti-LRPAP1 Antibody (6M356) can be used in WB,ELISA,ICC/IF,IP.
Anti-LRPAP1 Antibody (6M356)
Anti-LRPAP1 Antibody (6M356)
😃Good
Catalog No. TMAY-01642Alias RAP, low density lipoprotein receptor-related protein associated protein 1, HBP44, C77774, AU042172, AI790446, AA617339
Anti-LRPAP1 Antibody (6M356) is a Rabbit antibody targeting LRPAP1. Anti-LRPAP1 Antibody (6M356) can be used in WB,ELISA,ICC/IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-LRPAP1 Antibody (6M356) is a Rabbit antibody targeting LRPAP1. Anti-LRPAP1 Antibody (6M356) can be used in WB,ELISA,ICC/IF,IP. |
| Alias | RAP, low density lipoprotein receptor-related protein associated protein 1, HBP44, C77774, AU042172, AI790446, AA617339 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6M356 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human LRPAP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LRPAP1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Anti-LRPAP1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: U87-MG Whole Cell Lysate. -Lane B: PC-3 Whole Cell Lysate. -Lane C: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:41 kDa. -Observed band size:41 kDa. 3. LRPAP1 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -2 µL anti-LRPAP1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-LRPAP1 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 41 kDa. -Observed band size: 41 kDa |
| Application | WB,ELISA,ICC/IF,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human LRPAP1 / A2MRAP (rh LRPAP1; TMPY-01233; Tyr 35-Leu 357; NP_002328.1). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Receptor-associated protein (RAP) is a molecular chaperone for low-density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low-density lipoprotein receptor-related protein-associated protein 1, RAP, and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low-density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on the cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330) and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiasis. The genetic variations at the LRPAP1 locus may modulate Alzheimer's disease (AD) phenotype beyond risk for disease. Also, the variation in the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI). |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human LRPAP1 protein (TMPY-01233) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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