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Anti-PD-L2 Antibody (2N448) is a Mouse antibody targeting PD-L2. Anti-PD-L2 Antibody (2N448) can be used in ELISA, WB, IHC, IF, FCM, IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Mouse Monoclonal Application: ELISA, WB, IHC, IF, FCM, IP Reactivity: Human |
Alias | PD-L2, PDL2, PDCD1LG2, PDCD1L2, CD273, PDCD1 ligand 2, Butyrophilin B7-DC, Btdc, bA574F11.2, B7DC |
Ig Type | IgG2b |
Clone | 2N448 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate -All lanes PD-L2 antibody at 1:2000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 31,21 KDa -Observed band size: 45-50 KDa -Exposure time:5min 2. Western Blot -Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg -All lanes: PD-L2 antibody at 1:2000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 31,21 KDa -Observed band size: 45-50 KDa -Exposure time:5min 3. Western Blot -Positive WB detected in: 15μg hela whole cell lysate PD-L2 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 31,21 KDa -Observed band size: 45-50 KDa -Exposure time:5min 4. IHC image of TMAH-00872 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 5. Immunofluorescence staining of HepG2 cells with TMAH-00872 at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). 6. Immunofluorescence staining of Hela cells with TMAH-00872 at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). 7. Immunofluorescence staining of Raji cells with TMAH-00872 at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). 8. Immunofluorescence staining of U251 cells with TMAH-00872 at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). 9. Overlay histogram showing 293 cells transfected with PD-L2 stained with TMAH-00872 (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2µg/1*106cells) for 1 h at 4°C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (2µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. 10. Immunoprecipitating PD-L2 in Hela whole cell lysate -Lane 1: Mouse control IgG instead of TMAH-00872 in Hela whole cell lysate -Lane 2: TMAH-00872 (2µl) + Hela whole cell lysate (500µg) -Lane 3: Hela whole cell lysate (20µg) For western blotting, the blot was detected with TMAH-00872 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 |
Application | ELISA, WB, IHC, IF, FCM, IP |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | [Isoform 3]: Secreted.; [Isoform 2]: Endomembrane system; Single-pass type I membrane protein.; [Isoform 1]: Cell membrane; Single-pass type I membrane protein. |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Purity | >95% |
Research Background | Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human Programmed cell death 1 ligand 2 Protein (21-118AA) |
Antigen Species | Human |
Gene ID | 80380 |
Uniprot ID | |
Biology Area | Immunology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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