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Neuropilin-1 Protein, Human, Recombinant (Avi & His), Biotinylated

(Synonyms: VEGF165R, NRP, NP1, Neuropilin-1, neuropilin 1, CD304, BDCA4) Copy Product Info

Synonyms: VEGF165R, NRP, NP1, Neuropilin-1, neuropilin 1, CD304, BDCA4

Catalog No. TMPY-05585 Copy Product Info
Neuropilin-1 Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 73.1 kDa and the accession number is O14786-2.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$1677-10 days7-10 days
10 μg$2777-10 days7-10 days
20 μg$4627-10 days7-10 days
50 μg$7467-10 days7-10 days
100 μg$1,2307-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized VEGF165 at 10 μg/mL (100 μL/well) can bind Human Neuropilin-1, Biotinylated , the EC50 of Human Neuropilin-1, Biotinylated is 300-600 ng/mL.
Description
Neuropilin-1 Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 73.1 kDa and the accession number is O14786-2.
Species
Human
Expression System
HEK293 Cells
TagHis, Avi
Accession NumberO14786-2
ConstructionA DNA sequence encoding the human NRP1 (NP_001019799.1) (Met1-Lys644) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Phe 22
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsVEGF165R, NRP, NP1, Neuropilin-1, neuropilin 1, CD304, BDCA4
Research Background
Chemical Properties
Molecular Weight73.1 kDa (predicted)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: Neuropilin-1 Protein, Human, Recombinant (Avi & His), Biotinylated chemical structure | Neuropilin-1 Protein, Human, Recombinant (Avi & His), Biotinylated molecular weight