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Anti-FPGS Antibody (7Z220)
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Catalog No. TMAH-00457
Alias Tetrahydrofolylpolyglutamate synthase, Tetrahydrofolate synthase, mitochondrial, Folylpolyglutamate synthase, Folylpoly-gamma-glutamate synthetase, EC 6.3.2.17
Anti-FPGS Antibody (7Z220) is an antibody targeting FPGS. Anti-FPGS Antibody (7Z220) can be used in ELISA, FCM.
Anti-FPGS Antibody (7Z220)
Anti-FPGS Antibody (7Z220)
😃Good
Catalog No. TMAH-00457Alias Tetrahydrofolylpolyglutamate synthase, Tetrahydrofolate synthase, mitochondrial, Folylpolyglutamate synthase, Folylpoly-gamma-glutamate synthetase, EC 6.3.2.17
Anti-FPGS Antibody (7Z220) is an antibody targeting FPGS. Anti-FPGS Antibody (7Z220) can be used in ELISA, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, FCM Reactivity: Human |
| Alias | Tetrahydrofolylpolyglutamate synthase, Tetrahydrofolate synthase, mitochondrial, Folylpolyglutamate synthase, Folylpoly-gamma-glutamate synthetase, EC 6.3.2.17 |
| Ig Type | Rabbit IgG |
| Clone | 7Z220 |
| Reactivity | Human |
| Verified Activity | Overlay Peak curve showing HepG2 cells stained with TMAH-00457 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, FCM |
| Recommended Dose | FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | [Isoform 1]: Mitochondrion inner membrane. Mitochondrion matrix.; [Isoform 2]: Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human FPGS |
| Antigen Species | Human |
| Gene ID | 2356 |
| Uniprot ID | |
| Biology Area | Metabolism, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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