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Anti-MCP-3/CCL7 Antibody (4B549)
🥰Excellent
Catalog No. TMAY-02144
Anti-MCP-3/CCL7 Antibody (4B549) is a Mouse antibody targeting MCP-3/CCL7. Anti-MCP-3/CCL7 Antibody (4B549) can be used in ELISA,FCM,ICC/IF.
Anti-MCP-3/CCL7 Antibody (4B549)
Anti-MCP-3/CCL7 Antibody (4B549)
🥰Excellent
Catalog No. TMAY-02144
Anti-MCP-3/CCL7 Antibody (4B549) is a Mouse antibody targeting MCP-3/CCL7. Anti-MCP-3/CCL7 Antibody (4B549) can be used in ELISA,FCM,ICC/IF.
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Product Introduction
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-MCP-3/CCL7 Antibody (4B549) is a Mouse antibody targeting MCP-3/CCL7. Anti-MCP-3/CCL7 Antibody (4B549) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 4B549 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human CCL7 in SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCL7 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human CCL7 expression on THP-1 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CCL7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CCL7 / MCP3 (rh CCL7 / MCP3; NP_006264.2Gln 24-Leu 99). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 7(CCL7), also known as MCP-3, is a isform of the C-C chemokine subfamily of the chemokine family which is produced by certain tumor cells and by macrophages. It also own two adjacent N-terminal cysteine residues. Chemokine ligand 7(CCL7) spacifically attracts monocytes, and regulates macrophage function. |
| Conjucates | Unconjugated |
| Others Formats | PE |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Human CCL7 / MCP3 protein |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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