1. Immobilized mIL13RA2-mFc(mIgG2a) at 10 μg/mL (100 μL/well) can bind IL13/Biotin , the EC50 of IL13/Biotin is 20-50 ng/mL. 2. Measured by its ability to inhibit mIL13-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-30ng/mL in the presence of 4 ng/mL of mIL13.

IL-13RA2 Protein, Mouse, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 62.84 kDa and the accession number is O88786-1.

Mouse

HEK293 Cells

interleukin 13 receptor, α2,interleukin 13 receptor, alpha 2,CD213a2

A DNA sequence encoding the mouse IL13RA2 (NP_032382.1) (Met1-Lys334) was expressed with the Fc region of mouse IgG2a at the C-terminus. Predicted N terminal: Leu 22

> 95 % as determined by SDS-PAGE.

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High-Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors.