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Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. IL-2 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 20.1 kDa and the accession number is P04351-1.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 μg | $91 | 7-10 days | 7-10 days | |
| 10 μg | $148 | 7-10 days | 7-10 days | |
| 20 μg | $245 | 7-10 days | 7-10 days | |
| 50 μg | $483 | 7-10 days | 7-10 days | |
| 100 μg | $813 | 7-10 days | 7-10 days | |
| 200 μg | $1,470 | 7-10 days | 7-10 days | |
| 500 μg | $3,250 | 7-10 days | 7-10 days |
| Biological Activity | Immobilized Mouse IL-2 R alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 65.2ng/ml determined by ELISA. |
| Description | Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. IL-2 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 20.1 kDa and the accession number is P04351-1. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | N-His-Avi |
| Accession Number | P04351-1 |
| Synonyms | TCGF,T-cell growth factor,IL2,Aldesleukin |
| Construction | Ala21-Gln169 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Molecular Weight | 20.1 kDa (predicted). Due to glycosylation, the protein migrates to 27-30 kDa based on Tris-Bis PAGE result. |
| Endotoxin | < 1 EU/μg by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, Lyophilized powders are shipping with blue ice. |
| Research Background | Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. |
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