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Anti-ANGPT2/Angiopoietin-2 Antibody (6U152)
😃Good
Catalog No. TMAH-00059
Alias Tie2-ligand, ANGPT2, angiopoitin 2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2, angiopoietin 2, ANG-2, ANG2, AGPT2
Anti-ANGPT2/Angiopoietin-2 Antibody (6U152) is an antibody targeting ANGPT2/Angiopoietin-2. Anti-ANGPT2/Angiopoietin-2 Antibody (6U152) can be used in ELISA, IHC.
Anti-ANGPT2/Angiopoietin-2 Antibody (6U152)
Anti-ANGPT2/Angiopoietin-2 Antibody (6U152)
😃Good
Catalog No. TMAH-00059Alias Tie2-ligand, ANGPT2, angiopoitin 2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2, angiopoietin 2, ANG-2, ANG2, AGPT2
Anti-ANGPT2/Angiopoietin-2 Antibody (6U152) is an antibody targeting ANGPT2/Angiopoietin-2. Anti-ANGPT2/Angiopoietin-2 Antibody (6U152) can be used in ELISA, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IHC Reactivity: Human |
| Alias | Tie2-ligand, ANGPT2, angiopoitin 2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2, angiopoietin 2, ANG-2, ANG2, AGPT2 |
| Ig Type | Rabbit IgG |
| Clone | 6U152 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00059 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. IHC image of TMAH-00059 diluted at 1:50 and staining in paraffin-embedded human Liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody 3. Western Blot -Positive WB detected in: Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), U251 whole cell lysate(30µg),Mouse Heart tissue lysate(30µg),Mouse Brain tissue lysate(30µg),Rat Heart tissue lysate(30µg),Rat Brain tissue lysate(30µg) -All lanes: ANGPT2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/20000 dilution -Predicted band size: 57 kDa -Observed band size: 57 kDa -Exposure time: 120s 4. IHC image of TMAH-00059 diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody |
| Application | ELISA, IHC |
| Recommended Dose | IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Secreted. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Angiopoietin 2 |
| Antigen Species | Human |
| Gene ID | 285 |
| Uniprot ID | |
| Biology Area | Cardiovascular |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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