Antibody Type: Mouse Monoclonal

Application: WB, ELISA, FCM, IP

Reactivity: Human

Mouse IgG2a

7S22

Human

1. Anti-PPIL1 mouse monoclonal antibody at 1:500 dilution.
-Lane A: U87MG Whole Cell Lysate.
-Lane B: K562 Whole Cell Lysate.
-Lane C: Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:18 kDa.
-Observed band size:19 kDa(We are unsure as to the identity of these extra bands.)
2. PPIL1 was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-Lane B:0.5 mg U87MG Whole Cell Lysate.
-2 µL anti-PPIL1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-PPIL1 mouse monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 18 kDa.
-Observed band size: 18 kDa.
3. Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

WB,ELISA,FCM,IP

WB: 1:500-1:2000; ELISA: 1:1000-1:2000; FCM: 1:25-1:100; IP: 4-6 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Protein: Human PPIL1 protein (TMPY-03285)

Human