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Anti-PPIL1 Antibody (7S22)

😃Good
Catalog No. TMAY-02694
Alias PPIase, peptidylprolyl isomerase (cyclophilin)-like 1, hCyPX, CYPL1, CGI-124

Anti-PPIL1 Antibody (7S22) is a Mouse antibody targeting PPIL1. Anti-PPIL1 Antibody (7S22) can be used in WB,ELISA,FCM,IP.

Anti-PPIL1 Antibody (7S22)

Anti-PPIL1 Antibody (7S22)

😃Good
Catalog No. TMAY-02694Alias PPIase, peptidylprolyl isomerase (cyclophilin)-like 1, hCyPX, CYPL1, CGI-124
Anti-PPIL1 Antibody (7S22) is a Mouse antibody targeting PPIL1. Anti-PPIL1 Antibody (7S22) can be used in WB,ELISA,FCM,IP.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PPIL1 Antibody (7S22) is a Mouse antibody targeting PPIL1. Anti-PPIL1 Antibody (7S22) can be used in WB,ELISA,FCM,IP.
AliasPPIase, peptidylprolyl isomerase (cyclophilin)-like 1, hCyPX, CYPL1, CGI-124
Ig Type
Monoclonal Mouse IgG2a
Clone
7S22
Reactivity
Human
Verified Activity
1. Anti-PPIL1 mouse monoclonal antibody at 1:500 dilution.

-Lane A: U87MG Whole Cell Lysate.

-Lane B: K562 Whole Cell Lysate.

-Lane C: Hela Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:18 kDa.

-Observed band size:19 kDa(We are unsure as to the identity of these extra bands.)

2. PPIL1 was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg U87MG Whole Cell Lysate.

-2 µL anti-PPIL1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-PPIL1 mouse monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 18 kDa.

-Observed band size: 18 kDa.

3. Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
WB,ELISA,FCM,IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:1000-1:2000; FCM: 1:25-1:100; IP: 4-6 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPIL1 (rh PPIL1; TMPY-03285; Q9Y3C6; Met1-Gly166).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundPPIL1 is a member of the cyclophilin family. Cyclophilins are well conserved and ubiquitous. Members of cyclophilin family take a significant part in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. PPIL1 is a peptidylprolyl isomerase(PPIase). It increases the folding of proteins and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIL1 is involved in proliferation of cancer cells through modulation of phosphorylation of stathmin. It is a novel molecular target for colon-cancer therapy.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human PPIL1 protein (TMPY-03285)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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