Shopping Cart
- Remove All
- Your shopping cart is currently empty
Anti-PPIL1 Antibody (7S22) is a Mouse antibody targeting PPIL1. Anti-PPIL1 Antibody (7S22) can be used in WB,ELISA,FCM,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $199 | 7-10 days |
Description | Anti-PPIL1 Antibody (7S22) is a Mouse antibody targeting PPIL1. Anti-PPIL1 Antibody (7S22) can be used in WB,ELISA,FCM,IP. |
Alias | PPIase, peptidylprolyl isomerase (cyclophilin)-like 1, hCyPX, CYPL1, CGI-124 |
Ig Type | Monoclonal Mouse IgG2a |
Clone | 7S22 |
Reactivity | Human |
Verified Activity | 1. Anti-PPIL1 mouse monoclonal antibody at 1:500 dilution. -Lane A: U87MG Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:18 kDa. -Observed band size:19 kDa(We are unsure as to the identity of these extra bands.) 2. PPIL1 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg U87MG Whole Cell Lysate. -2 µL anti-PPIL1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PPIL1 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 18 kDa. -Observed band size: 18 kDa. 3. Flow cytometric analysis of Human PPIL1 expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PPIL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
Application | WB,ELISA,FCM,IP |
Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; FCM: 1:25-1:100; IP: 4-6 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PPIL1 (rh PPIL1; TMPY-03285; Q9Y3C6; Met1-Gly166). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | PPIL1 is a member of the cyclophilin family. Cyclophilins are well conserved and ubiquitous. Members of cyclophilin family take a significant part in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. PPIL1 is a peptidylprolyl isomerase(PPIase). It increases the folding of proteins and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIL1 is involved in proliferation of cancer cells through modulation of phosphorylation of stathmin. It is a novel molecular target for colon-cancer therapy. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human PPIL1 protein (TMPY-03285) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.