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Anti-ICAM-1/CD54 Antibody (3B313)
🥰Excellent
Catalog No. TMAY-03695
Anti-ICAM-1/CD54 Antibody (3B313) is a Rabbit antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody (3B313) can be used in ELISA(Cap),FCM,ICC/IF.
Anti-ICAM-1/CD54 Antibody (3B313)
Anti-ICAM-1/CD54 Antibody (3B313)
🥰Excellent
Catalog No. TMAY-03695
Anti-ICAM-1/CD54 Antibody (3B313) is a Rabbit antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody (3B313) can be used in ELISA(Cap),FCM,ICC/IF.
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Product Introduction
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Related Conjugates and Formulations
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Chemical Properties
| Description | Anti-ICAM-1/CD54 Antibody (3B313) is a Rabbit antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody (3B313) can be used in ELISA(Cap),FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 3B313 |
| Reactivity | Rat |
| Verified Activity | 1. Flow cytometric analysis of Rat ICAM1 expression on SD rat splenocytes. Cells were stained with purified anti-Rat ICAM1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of rat ICAM1 in rat splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-rat ICAM1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). |
| Application | ELISA(Cap),FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Rat ICAM-1 / CD54 (rR ICAM-1 / CD54; TMPY-02017; Q00238-1; Met1-Thr493). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
| Conjucates | Unconjugated |
| Others Formats | APC/FITC/PE |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Rat ICAM-1 / CD54 protein (TMPY-02017) |
| Antigen Species | Rat |
| Biology Area | Neuroinflammation |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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