4-Di-1-ASP

4-Di-1-ASP is a positively charged fluorescent dye used to label glioma cells in live brain tissue and can also be used to observe mitochondria in live cells, with λex λem = 475 606 nm.

5-ROX (5-Carboxy-X-rhodamine), a rhodamine dye, exhibits strong fluorescence in aqueous buffer with an excitation wavelength (λexit) of 580 nm (ε = 3.6×10^4 (M·cm)) and an emission wavelength (λemit) of 604 nm (ϕ = 0.94).

(E)-4-Dimethylamino-N-methylstilbazolium iodide (Py-NMe2) is a styrene-based dye employed in sensing various double-stranded DNA RNA sequences using fluorescence and circular dichroism (CD) techniques. Its absorption maximum is 450 nm, and its emission maximum is 615 nm.

7-(2,4-Dinitrophenoxy)-4-methyl-2H-chromen-2-one (Compound 1) serves as a fluorescent probe for detecting hydrogen sulfide (H2S). It is characterized by a low detection limit (4×10^-6 mol L), high selectivity, and great sensitivity. At a concentration of 150 µg mL, it exhibits negligible cytotoxicity. The excitation wavelength is 331 nm, while in DMSO solvent, it emits at approximately 385 nm; upon addition of HS-, the fluorescence intensity significantly increases around 392 nm.

Asante potassium green-1 AM is a membrane-permeable, acetoxymethyl (AM) ester-form fluorescent potassium indicator that emits in the yellow-green spectrum.Asante potassium green-1 AM is specifically tailored to measure dynamic changes in potassium ion concentrations in intracellular and extracellular environments characterized by high potassium concentrations, as it possesses a lower potassium binding affinity compared to other indicators like IPG-2 and IPG-4.

8-(4-ethynylphenyl)-1,3,5,7-tetramethyl BODIPY is an organic fluorescent dye characterized by an ethynylphenyl group at the 8-position of the boron dipyrromethene (BODIPY) structure and methyl substituents at the 1, 3, 5, and 7 positions. It boasts a high fluorescence quantum yield and specific chemical properties, making it useful for applications such as fluorescence imaging.

8-(4-bromophenyl)-1,3,5,7-tetramethyl-BODIPY is a derivative based on the BODIPY structure, featuring four methyl groups at positions 1, 3, 5, and 7, with a para-bromophenyl group attached at position 8. This compound finds extensive use in fields such as bioimaging, chemical analysis, and materials science, serving as a fluorescent probe for imaging biological samples, detecting specific chemicals, or in the development of optical materials.

GSK-3βprobe-1 (10c) is a GSK-3β probe, characterized by an excitation wavelength of 341 nm and an emission wavelength of 574 nm, with a molar absorptivity of 3.51 × 10^4 L/mol·cm−1. This compound is utilized in Alzheimer's disease research.

Lumogallion (4-Chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid) is an azo reagent used in the determination of metal ions, such as the research of V, Fe, and Al.

FD&C Yellow No. 5 Aluminum Lake is a synthetic lemon yellow azo dye primarily used as a food coloring.

cis-Parinaric acid is a naturally occurring polyunsaturated fatty acid containing an unusual conjugated (Z,E,E,Z) tetraene. This chromophore provides for a natural fluorescence at 432 nm with an excitation wavelength at 320 nm. cis-Parinaric acid occurs naturally in the seeds of the Makita tree, a tropical rainforest tree indigenous to Fiji. Makita seeds are inedible, and this toxicity may be due at least in part to the unstable conjugated fatty acids, including cis-parinaric acid, contained within the seed. cis-Parinaric acid has been used for the measurement of phospholipase activity, lipase activity, and as an indicator of lipid peroxidation.[1][2][3][4]

STY-BODIPY is a styrene-conjugated fluorogenic probe for radical-trapping antioxidant (RTA) activity.1 Co-autoxidation of the STY-BODIPY signal carrier and a hydrocarbon co-substrate can be quantified by monitoring the loss of absorbance at 571 nm. STY-BODIPY has been used to measure the activity of RTAs, as well as the kinetics and stoichiometry of RTA reactions in cell-free assays.1,2,3References1. Haidasz, E.A., Van Kessel, A.T.M., and Pratt, D.A. A continuous visible light spectrophotometric approach to accurately determine the reactivity of radical-trapping antioxidants. J. Org. Chem. 81(3), 737-744 (2016).2. Shah, R., Shchepinov, M.S., and Pratt, D.A. Resolving the role of lipoxygenases in the initiation and execution of ferroptosis. ACS Cent. Sci. 4(3), 387-396 (2018).3. Chauvin, J.-P.R., Haidasz, E.A., Griesser, M., et al. Polysulfide-1-oxides react with peroxyl radicals as quickly as hindered phenolic antioxidants and do so by a surprising concerted homolytic substitution. Chem. Sci. 7(10), 6347-6356 (2016). STY-BODIPY is a styrene-conjugated fluorogenic probe for radical-trapping antioxidant (RTA) activity.1 Co-autoxidation of the STY-BODIPY signal carrier and a hydrocarbon co-substrate can be quantified by monitoring the loss of absorbance at 571 nm. STY-BODIPY has been used to measure the activity of RTAs, as well as the kinetics and stoichiometry of RTA reactions in cell-free assays.1,2,3 References1. Haidasz, E.A., Van Kessel, A.T.M., and Pratt, D.A. A continuous visible light spectrophotometric approach to accurately determine the reactivity of radical-trapping antioxidants. J. Org. Chem. 81(3), 737-744 (2016).2. Shah, R., Shchepinov, M.S., and Pratt, D.A. Resolving the role of lipoxygenases in the initiation and execution of ferroptosis. ACS Cent. Sci. 4(3), 387-396 (2018).3. Chauvin, J.-P.R., Haidasz, E.A., Griesser, M., et al. Polysulfide-1-oxides react with peroxyl radicals as quickly as hindered phenolic antioxidants and do so by a surprising concerted homolytic substitution. Chem. Sci. 7(10), 6347-6356 (2016).

bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.[1] Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.[2] bis-ANS has an excitation maxima of 390 nm.[3] It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices.4 It also potently inhibits microtubule assembly.[5],[6]

7-Azido-4-methylcoumarin (7-azido-4-methyl-2H-chromen-2-one) is a fluorescent photoactive probe used in the study of human phenol sulfotransferase (SULT1A1 or P-PST-1) substrate binding sites through photoaffinity labeling.

7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) is a thiol-reactive fluorogenic probe.1It has been used to quantify the levels of homocysteine, cysteine, and cysteamine in human plasma.2SBD-F displays excitation emission maxima of 380 515 nm, respectively.1 1.Imai, K., Toyo'oka, T., and Watanabe, Y.A novel fluorogenic reagent for thiols: Ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonateAnal. Biochem.128(2)471-473(1983) 2.Ichinose, S., Nakamura, M., Maeda, M., et al.A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasmaBiomed. Chromatogr.23(9)935-939(2009)

Z-(L-Arg)-AMC is a fluorogenic substrate for trypsin, cathepsin B, and cathepsin H.1,2Upon enzymatic cleavage by trypsin, cathepsin B, or cathepsin H, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify trypsin, cathepsin B, and cathepsin H activity. AMC displays excitation emission maxima of 340-360 440-460 nm, respectively. 1.Zimmerman, M., Ashe, B., Yurewicz, E.C., et al.Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substratesAnal. Biochem.78(1)47-51(1977) 2.Brindley, P.J., Kalinna, B.H., Dalton, J.P., et al.Proteolytic degradation of host hemoglobin by schistosomesMol. Biochem. Parasitol.89(1)1-9(1997)

Ac-ANW-AMC is a fluorogenic substrate for the β5i LMP7 subunit of the 20S immunoproteasome. [1] Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released, and its fluorescence quantifies the activity of the β5i LMP7 subunit. AMC displays excitation emission maxima of 340-360 440-460 nm, respectively.

4-Methylumbelliferyl-β-D-galactoside (MUG) is a fluorogenic substrate for β-galactosidase (β-gal).1Hydrolysis of MUG by β-gal releases the fluorescent moiety 4-MU, which displays a pH-dependent excitation maximum of 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively, and an emission maximum between 445-454 nm.2MUG has been used to detect β-gal activity in intact bacteria, yeast, and mammalian cells.1 1.Vidal-Aroca, F., Giannattasio, M., Brunelli, E., et al.One-step high-throughput assay for quantitative detection of β-galactosidase activity in intact Gram-negative bacteria, yeast, and mammalian cellsBiotechniques40(4)433-434(2006) 2.Zhi, H., Wang, J., Wang, S., et al.Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsuleJ. Spectrosc.1(1)147128(2013)

Biotin-11-dUTP [tetra(triethylammonium) salt] [(2S,3S,4R,5R,6R)-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxy-6-(hydroxymethyl)-2-[(3aR,4S,6R,6aS)-2-oxohexahydro-2H-thieno[3,4-d]imidazol-4-yl]-tetrahydro-2H-furo[3,2-d][1,3,2]dioxaphosphorin-3-yl]phosphonate] is used as a fluorescent substitute for dTTP.

4,4'-((4-Iminocyclohexa-2,5-dien-1-ylidene)methylene)dianiline hydrochloride is a useful organic compound for research related to life sciences. The catalog number is T64740 and the CAS number is 569-61-9.

7-Dimethylaminocoumarin-4-acetic acid, catalog number T64950, CAS number 80883-54-1, is a valuable organic compound for life sciences research.

N-(4-(Bis(4-(diethylamino)phenyl)methylene)cyclohexa-2,5-dien-1-ylidene)-N-ethylethanaminium chloride is a useful organic compound for research related to life sciences. The catalog number is T65162 and the CAS number is 2390-59-2.

7-(Diethylamino)-4-methyl-2H-chromen-2-one is a useful organic compound for research related to life sciences. The catalog number is T66262 and the CAS number is 91-44-1.

7-Hydroxy-4-methyl-2(1H)-quinolone (compound 2b), a fluorescent hydroxylated derivative, serves as a probe for detecting hydroxyl radicals associated with DNA damage [1]. It should be protected from light for preservation.