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PDK4 Protein, Mouse, Recombinant (His & GST)

Catalog No. TMPY-04445
Synonyms: AV005916, pyruvate dehydrogenase kinase 4

Pyruvate dehydrogenase kinase 4 (PDK4) is a mitochondrial protein that regulates the TCA cycle.PDK4, a vital mitochondrial protein, controls the switch between glycolysis and oxidative phosphorylation based upon nutrient availability.Pyruvate dehydrogenase kinase 4 (PDK4) mRNA has been reported as an up-regulated gene in the heart and skeletal muscle of carnitine-deficient juvenile visceral steatosis (JVS) mice under fed conditions. PDK4 plays an important role in the inhibition of glucose oxidation via the phosphorylation of pyruvate dehydrogenase complex (PDC).PDK4 gene expression is stimulated by thyroid hormone (T(3)), glucocorticoids, and long chain fatty acids.

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PDK4 Protein, Mouse, Recombinant (His & GST)
Pack Size Availability Price/USD Quantity
50 μg 5 days $ 498.00
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Biological Description
Technical Params
Product Properties
Description Pyruvate dehydrogenase kinase 4 (PDK4) is a mitochondrial protein that regulates the TCA cycle.PDK4, a vital mitochondrial protein, controls the switch between glycolysis and oxidative phosphorylation based upon nutrient availability.Pyruvate dehydrogenase kinase 4 (PDK4) mRNA has been reported as an up-regulated gene in the heart and skeletal muscle of carnitine-deficient juvenile visceral steatosis (JVS) mice under fed conditions. PDK4 plays an important role in the inhibition of glucose oxidation via the phosphorylation of pyruvate dehydrogenase complex (PDC).PDK4 gene expression is stimulated by thyroid hormone (T(3)), glucocorticoids, and long chain fatty acids.
Species Mouse
Expression System Baculovirus-Insect Cells
Tag His,GST
Accession Number O70571
Synonyms AV005916, pyruvate dehydrogenase kinase 4
Construction A DNA sequence encoding the mouse PDK4 (O70571) (Met1-Val412) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity > 85 % as determined by SDS-PAGE
Molecular Weight 74.4 kDa (predicted)
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.

Research Background Pyruvate dehydrogenase kinase 4 (PDK4) is a mitochondrial protein that regulates the TCA cycle.PDK4, a vital mitochondrial protein, controls the switch between glycolysis and oxidative phosphorylation based upon nutrient availability.Pyruvate dehydrogenase kinase 4 (PDK4) mRNA has been reported as an up-regulated gene in the heart and skeletal muscle of carnitine-deficient juvenile visceral steatosis (JVS) mice under fed conditions. PDK4 plays an important role in the inhibition of glucose oxidation via the phosphorylation of pyruvate dehydrogenase complex (PDC).PDK4 gene expression is stimulated by thyroid hormone (T(3)), glucocorticoids, and long chain fatty acids.

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Keywords

PDK4 Protein, Mouse, Recombinant (His & GST) AV 005916 AV005916 AV-005916 pyruvate dehydrogenase kinase 4 recombinant recombinant-proteins proteins protein

 

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