3',4'-Dihydroxyflavonol (DiOHF) is an effective antioxidant that reduces ROS and superoxide production in the mesenteric arteries of diabetic rats, increases total eNOS expression, and improves nitric oxide (NO) activity.

3',4'-Dihydroxyflavonol (DiOHF) exhibits significant vascular protective effects under oxidative stress conditions, maintaining the biological activity of nitric oxide (NO) through multiple mechanisms. This compound can effectively alleviate the vasoconstrictive response of the tertiary branches of the rat mesenteric artery through Ca²⁺ desensitization, thereby reducing vascular permeability. At the same time, DiOHF significantly improves NO activity and endothelial function in pathological conditions such as diabetes by inhibiting Nox2-dependent superoxide production and preventing eNOS uncoupling. These synergistic effects make DiOHF a potential vascular function regulator, especially in pathological conditions with elevated reactive oxygen species levels, showing important therapeutic value. [1]