Anti-CLEC7A Polyclonal Antibody 2 is a Rabbit antibody targeting CLEC7A. Anti-CLEC7A Polyclonal Antibody 2 can be used in FCM,WB.

IgG

Human,Mouse,Rat

1. Sample: Lung (Mouse) lysate at 30 μg;
Primary: Anti-beta Glucan Receptor (TMAB-00449) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1:5000 dilution;
Predicted band size: 28 kDa
Observed band size: 28 kDa
2. Sample:
Spleen (Mouse) Lysate at 40 μg
Thymus (Mouse) Lysate at 40 μg
Primary: Anti-beta Glucan Receptor (TMAB-00449) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
3. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-beta Glucan Receptor antibody (TMAB-00449)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Blank control: RAW264.7. Primary Antibody (green line): Rabbit Anti-Iba1 antibody (TMAB-00449)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-Rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti-beta Glucan Receptor (TMAB-00449) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28,47 kDa

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human CLEC7A

Human

CLEC7A

C-type lectin domain family 7 member A

Macrophage / Inflamm.

Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity).