Antibody Type: Rabbit Polyclonal

Application: WB,FCM

Reactivity: Human,Mouse,Rat

IgG

Human,Mouse,Rat

1. Sample: Lung (Mouse) lysate at 30 μg;
Primary: Anti-beta Glucan Receptor (TMAB-00449) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1:5000 dilution;
Predicted band size: 28 kDa
Observed band size: 28 kDa
2. Sample:
Spleen (Mouse) Lysate at 40 μg
Thymus (Mouse) Lysate at 40 μg
Primary: Anti-beta Glucan Receptor (TMAB-00449) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
3. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-beta Glucan Receptor antibody (TMAB-00449)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. Blank control: RAW264.7. Primary Antibody (green line): Rabbit Anti-Iba1 antibody (TMAB-00449)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-Rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti-beta Glucan Receptor (TMAB-00449) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28,47 kDa

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human CLEC7A

Human

64581

Macrophage / Inflamm.