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Anti-NOX4 Polyclonal Antibody

😃Good
Catalog No. TMAB-01255
Alias RENOX, Renal NAD(P)H-oxidase, NOX4, NADPH oxidase 4, Kidney superoxide-producing NADPH oxidase, Kidney oxidase-1 (KOX-1)

Anti-NOX4 Polyclonal Antibody is a Rabbit antibody targeting NOX4. Anti-NOX4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-NOX4 Polyclonal Antibody

Anti-NOX4 Polyclonal Antibody

😃Good
Catalog No. TMAB-01255Alias RENOX, Renal NAD(P)H-oxidase, NOX4, NADPH oxidase 4, Kidney superoxide-producing NADPH oxidase, Kidney oxidase-1 (KOX-1)
Anti-NOX4 Polyclonal Antibody is a Rabbit antibody targeting NOX4. Anti-NOX4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-NOX4 Polyclonal Antibody is a Rabbit antibody targeting NOX4. Anti-NOX4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasRENOX, Renal NAD(P)H-oxidase, NOX4, NADPH oxidase 4, Kidney superoxide-producing NADPH oxidase, Kidney oxidase-1 (KOX-1)
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Cow,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NADpHoxidase 4) Polyclonal Antibody, Unconjugated (TMAB-01255) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NADpHoxidase 4) Polyclonal Antibody, Unconjugated (TMAB-01255) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Lung (Mouse) Lysate at 40 μg
Lane 3: Bronchus (Mouse) Lysate at 40 μg
Lane 4: Heart (Mouse) Lysate at 40 μg
Lane 5: Kidney (Rat) Lysate at 40 μg
Lane 6: Lung (Rat) Lysate at 40 μg
Lane 7: Bronchus (Rat) Lysate at 40 μg
Primary: Anti-NADpHoxidase 4 (TMAB-01255) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 62 kDa
4. Blank control: 293T.
Primary Antibody (green line): Rabbit Anti-NADpHoxidase 4 antibody (TMAB-01255)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NADpHoxidase 4) Polyclonal Antibody, Unconjugated (TMAB-01255) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NADpHoxidase 4) Polyclonal Antibody, Unconjugated (TMAB-01255) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NADpHoxidase 4) Polyclonal Antibody, Unconjugated (TMAB-01255) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NADpHoxidase 4) Polyclonal Antibody, Unconjugated (TMAB-01255) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NADpHoxidase 4) Polyclonal Antibody, Unconjugated (TMAB-01255) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
10. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-NADpHoxidase 4 antibody (TMAB-01255)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Cell membrane. Note=May localize to plasma membrane and focal adhesions.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Nox-4
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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