CD20 is a 33-37 kDa non-glycosylated protein expressed on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 protein consists of four hydrophobic transmembrane domains, one intracellular and two extracellular domains (large and small loops) with both N- and C- termini residing within the cytosol. CD20 is also known to be physically coupled to major histocompatibility complex class II (MHCII), CD40 molecule, BCR, and the C-terminal src kinase-binding protein (CBP) that interacts with Src kinases such as LYN, FYN, and LCK. CD20 deficiency resulted in a reduced number of circulating memory B cells, reduced isotype switching of Ig, and decreased IgG antibody levels. In agreement with this observation, challenging the patient’s primary B cells in vitro using T-dependent and Tindependent antigens led to the normal proliferation and secretion of IgM but reduced production of IgG.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 μg | 5 days | $ 371.00 | |
50 μg | 5 days | $ 1,120.00 | |
500 μg | 5 days | $ 6,990.00 | |
1 mg | 5 days | $ 10,580.00 |
Description | CD20 is a 33-37 kDa non-glycosylated protein expressed on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 protein consists of four hydrophobic transmembrane domains, one intracellular and two extracellular domains (large and small loops) with both N- and C- termini residing within the cytosol. CD20 is also known to be physically coupled to major histocompatibility complex class II (MHCII), CD40 molecule, BCR, and the C-terminal src kinase-binding protein (CBP) that interacts with Src kinases such as LYN, FYN, and LCK. CD20 deficiency resulted in a reduced number of circulating memory B cells, reduced isotype switching of Ig, and decreased IgG antibody levels. In agreement with this observation, challenging the patient’s primary B cells in vitro using T-dependent and Tindependent antigens led to the normal proliferation and secretion of IgM but reduced production of IgG. |
Species | Human |
Expression System | Human Cells |
Tag | C-6His-Flag |
Accession Number | P11836 |
Synonyms | CD20, Membrane-spanning 4-domains subfamily A member 1, B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Bp35, MS4A1, B-lymphocyte antigen CD20 |
Amino Acid | Met1-Pro297 |
Construction | Recombinant Human B-lymphocyte antigen CD20 is produced by our Mammalian expression system and the target gene encoding Met1-Pro297 is expressed with a 6His, Flag tag at the C-terminus. |
Protein Purity | Greater than 80% as determined by reducing SDS-PAGE. (QC verified) |
Molecular Weight | 34-40 kDa, reducing conditions |
Endotoxin | Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test. |
Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES-Na, 150mM NaCl, 0.02%DDM, 0.004%CHS, 5%Glycerol, pH7.5. |
Stability & Storage |
Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
Shipping |
The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
Research Background | CD20 is a 33-37 kDa non-glycosylated protein expressed on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 protein consists of four hydrophobic transmembrane domains, one intracellular and two extracellular domains (large and small loops) with both N- and C- termini residing within the cytosol. CD20 is also known to be physically coupled to major histocompatibility complex class II (MHCII), CD40 molecule, BCR, and the C-terminal src kinase-binding protein (CBP) that interacts with Src kinases such as LYN, FYN, and LCK. CD20 deficiency resulted in a reduced number of circulating memory B cells, reduced isotype switching of Ig, and decreased IgG antibody levels. In agreement with this observation, challenging the patient’s primary B cells in vitro using T-dependent and Tindependent antigens led to the normal proliferation and secretion of IgM but reduced production of IgG. |
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CD20 Protein, Human, Recombinant (His & Flag) CD20 Membrane-spanning 4-domains subfamily A member 1 CD-20 B-lymphocyte surface antigen B1 Leukocyte surface antigen Leu-16 Bp35 CD 20 MS4A1 B-lymphocyte antigen CD20 recombinant recombinant-proteins proteins protein