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Anti-Caspase-8 subunit p18 Polyclonal Antibody is a Rabbit antibody targeting Caspase-8 subunit p18. Anti-Caspase-8 subunit p18 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Caspase-8 subunit p18 Polyclonal Antibody is a Rabbit antibody targeting Caspase-8 subunit p18. Anti-Caspase-8 subunit p18 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB. |
Synonyms | OTTHUMP00000206582, OTTHUMP00000206552, OTTHUMP00000165064, OTTHUMP00000165063, OTTHUMP00000165062, OTTHUMP00000163725, OTTHUMP00000163724, OTTHUMP00000163720, OTTHUMP00000163717, MORT1 associated CED 3 homolog, MGC78473, MCH5, MCH 5, MACH beta 1/2/3/4 protein, MACH alpha 1/2/3 protein, MACH, ICE like apoptotic protease 5, FLJ17672, FLICE, FADD-like ICE, FADD-homologous ICE/ced-3-like protease, FADD Like ICE, FADD Homologous ICE/CED3 Like Protease, FADD homologous ICE/CED 3 like protease, CED 3, Caspase-8 subunit p18, Caspase8, Caspase 8 apoptosis related cysteine peptidase, Caspase 8, CASP8, CASP 8, CAP4, CAP 4, Apoptotic protease Mch-5, Apoptotic protease Mch 5, Apoptotic cysteine protease, Apoptosis related cysteine peptidase, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, ALPS2B |
Ig Type | IgG |
Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (caspase-8 subunit p18) Polyclonal Antibody, Unconjugated (TMAB-00291) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-caspase-8 subunit p18 antibody (TMAB-00291), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test. 3. Sample: Jurkat (Human) Cell Lysate at 30 μg K562 (Human) Cell Lysate at 30 μg U87Mg (Human) Cell Lysate at 30 μg Primary: Anti-caspase-8 subunit p18 (TMAB-00291) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55/18 kDa Observed band size: 57 kDa |
Application | |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 1μg /Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. |
Tissue Specificity | Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Concentration | 1 mg/mL |
Immunogen | KLH conjugated synthetic peptide: human caspase-8 subunit p18 |
Antigen Species | Human |
Gene Name | CASP8 |
Gene ID | |
Protein Name | Caspase-8 |
Uniprot ID | |
Biology Area | Caspases,Metabolism,Necroptosis,Caspases,Caspases,Other proteases,Apoptosis |
Function | Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. |
Molecular Weight | Theoretical: 18/55 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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