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Anti-Caspase-8 subunit p18 Polyclonal Antibody

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Catalog No. TMAB-00291
Alias OTTHUMP00000206582, OTTHUMP00000206552, OTTHUMP00000165064, OTTHUMP00000165063, OTTHUMP00000165062, OTTHUMP00000163725, OTTHUMP00000163724, OTTHUMP00000163720, OTTHUMP00000163717, MORT1 associated CED 3 homolog, MGC78473, MCH5, MCH 5, MACH beta 1/2/3/4 protein, MACH alpha 1/2/3 protein, MACH, ICE like apoptotic protease 5, FLJ17672, FLICE, FADD-like ICE, FADD-homologous ICE/ced-3-like protease, FADD Like ICE, FADD Homologous ICE/CED3 Like Protease, FADD homologous ICE/CED 3 like protease, CED 3, Caspase-8 subunit p18, Caspase8, Caspase 8 apoptosis related cysteine peptidase, Caspase 8, CASP8, CASP 8, CAP4, CAP 4, Apoptotic protease Mch-5, Apoptotic protease Mch 5, Apoptotic cysteine protease, Apoptosis related cysteine peptidase, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, ALPS2B

Anti-Caspase-8 subunit p18 Polyclonal Antibody is a Rabbit antibody targeting Caspase-8 subunit p18. Anti-Caspase-8 subunit p18 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-Caspase-8 subunit p18 Polyclonal Antibody

Anti-Caspase-8 subunit p18 Polyclonal Antibody

😃Good
Catalog No. TMAB-00291Alias OTTHUMP00000206582, OTTHUMP00000206552, OTTHUMP00000165064, OTTHUMP00000165063, OTTHUMP00000165062, OTTHUMP00000163725, OTTHUMP00000163724, OTTHUMP00000163720, OTTHUMP00000163717, MORT1 associated CED 3 homolog, MGC78473, MCH5, MCH 5, MACH beta 1/2/3/4 protein, MACH alpha 1/2/3 protein, MACH, ICE like apoptotic protease 5, FLJ17672, FLICE, FADD-like ICE, FADD-homologous ICE/ced-3-like protease, FADD Like ICE, FADD Homologous ICE/CED3 Like Protease, FADD homologous ICE/CED 3 like protease, CED 3, Caspase-8 subunit p18, Caspase8, Caspase 8 apoptosis related cysteine peptidase, Caspase 8, CASP8, CASP 8, CAP4, CAP 4, Apoptotic protease Mch-5, Apoptotic protease Mch 5, Apoptotic cysteine protease, Apoptosis related cysteine peptidase, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, ALPS2B
Anti-Caspase-8 subunit p18 Polyclonal Antibody is a Rabbit antibody targeting Caspase-8 subunit p18. Anti-Caspase-8 subunit p18 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-Caspase-8 subunit p18 Polyclonal Antibody is a Rabbit antibody targeting Caspase-8 subunit p18. Anti-Caspase-8 subunit p18 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasOTTHUMP00000206582, OTTHUMP00000206552, OTTHUMP00000165064, OTTHUMP00000165063, OTTHUMP00000165062, OTTHUMP00000163725, OTTHUMP00000163724, OTTHUMP00000163720, OTTHUMP00000163717, MORT1 associated CED 3 homolog, MGC78473, MCH5, MCH 5, MACH beta 1/2/3/4 protein, MACH alpha 1/2/3 protein, MACH, ICE like apoptotic protease 5, FLJ17672, FLICE, FADD-like ICE, FADD-homologous ICE/ced-3-like protease, FADD Like ICE, FADD Homologous ICE/CED3 Like Protease, FADD homologous ICE/CED 3 like protease, CED 3, Caspase-8 subunit p18, Caspase8, Caspase 8 apoptosis related cysteine peptidase, Caspase 8, CASP8, CASP 8, CAP4, CAP 4, Apoptotic protease Mch-5, Apoptotic protease Mch 5, Apoptotic cysteine protease, Apoptosis related cysteine peptidase, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, ALPS2B
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (caspase-8 subunit p18) Polyclonal Antibody, Unconjugated (TMAB-00291) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-caspase-8 subunit p18 antibody (TMAB-00291), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
3. Sample:
Jurkat (Human) Cell Lysate at 30 μg
K562 (Human) Cell Lysate at 30 μg
U87Mg (Human) Cell Lysate at 30 μg
Primary: Anti-caspase-8 subunit p18 (TMAB-00291) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55/18 kDa
Observed band size: 57 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 1μg /Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human caspase-8 subunit p18
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Caspases,Metabolism,Necroptosis,Caspases,Caspases,Other proteases,Apoptosis
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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