Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-Caspase-8 subunit p18 Polyclonal Antibody

😃Good
Catalog No. TMAB-00291
Alias OTTHUMP00000206582, OTTHUMP00000206552, OTTHUMP00000165064, OTTHUMP00000165063, OTTHUMP00000165062, OTTHUMP00000163725, OTTHUMP00000163724, OTTHUMP00000163720, OTTHUMP00000163717, MORT1 associated CED 3 homolog, MGC78473, MCH5, MCH 5, MACH beta 1/2/3/4 protein, MACH alpha 1/2/3 protein, MACH, ICE like apoptotic protease 5, FLJ17672, FLICE, FADD-like ICE, FADD-homologous ICE/ced-3-like protease, FADD Like ICE, FADD Homologous ICE/CED3 Like Protease, FADD homologous ICE/CED 3 like protease, CED 3, Caspase-8 subunit p18, Caspase8, Caspase 8 apoptosis related cysteine peptidase, Caspase 8, CASP8, CASP 8, CAP4, CAP 4, Apoptotic protease Mch-5, Apoptotic protease Mch 5, Apoptotic cysteine protease, Apoptosis related cysteine peptidase, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, ALPS2B

Anti-Caspase-8 subunit p18 Polyclonal Antibody is a Rabbit antibody targeting Caspase-8 subunit p18. Anti-Caspase-8 subunit p18 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

Anti-Caspase-8 subunit p18 Polyclonal Antibody

Anti-Caspase-8 subunit p18 Polyclonal Antibody

😃Good
Catalog No. TMAB-00291Alias OTTHUMP00000206582, OTTHUMP00000206552, OTTHUMP00000165064, OTTHUMP00000165063, OTTHUMP00000165062, OTTHUMP00000163725, OTTHUMP00000163724, OTTHUMP00000163720, OTTHUMP00000163717, MORT1 associated CED 3 homolog, MGC78473, MCH5, MCH 5, MACH beta 1/2/3/4 protein, MACH alpha 1/2/3 protein, MACH, ICE like apoptotic protease 5, FLJ17672, FLICE, FADD-like ICE, FADD-homologous ICE/ced-3-like protease, FADD Like ICE, FADD Homologous ICE/CED3 Like Protease, FADD homologous ICE/CED 3 like protease, CED 3, Caspase-8 subunit p18, Caspase8, Caspase 8 apoptosis related cysteine peptidase, Caspase 8, CASP8, CASP 8, CAP4, CAP 4, Apoptotic protease Mch-5, Apoptotic protease Mch 5, Apoptotic cysteine protease, Apoptosis related cysteine peptidase, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, ALPS2B
Anti-Caspase-8 subunit p18 Polyclonal Antibody is a Rabbit antibody targeting Caspase-8 subunit p18. Anti-Caspase-8 subunit p18 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5277-10 days
Add to Cart
Add to Quotation
Bulk & Custom
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View More

Product Introduction

Bioactivity
Description
Anti-Caspase-8 subunit p18 Polyclonal Antibody is a Rabbit antibody targeting Caspase-8 subunit p18. Anti-Caspase-8 subunit p18 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.
SynonymsOTTHUMP00000206582, OTTHUMP00000206552, OTTHUMP00000165064, OTTHUMP00000165063, OTTHUMP00000165062, OTTHUMP00000163725, OTTHUMP00000163724, OTTHUMP00000163720, OTTHUMP00000163717, MORT1 associated CED 3 homolog, MGC78473, MCH5, MCH 5, MACH beta 1/2/3/4 protein, MACH alpha 1/2/3 protein, MACH, ICE like apoptotic protease 5, FLJ17672, FLICE, FADD-like ICE, FADD-homologous ICE/ced-3-like protease, FADD Like ICE, FADD Homologous ICE/CED3 Like Protease, FADD homologous ICE/CED 3 like protease, CED 3, Caspase-8 subunit p18, Caspase8, Caspase 8 apoptosis related cysteine peptidase, Caspase 8, CASP8, CASP 8, CAP4, CAP 4, Apoptotic protease Mch-5, Apoptotic protease Mch 5, Apoptotic cysteine protease, Apoptosis related cysteine peptidase, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, ALPS2B
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (caspase-8 subunit p18) Polyclonal Antibody, Unconjugated (TMAB-00291) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-caspase-8 subunit p18 antibody (TMAB-00291), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
3. Sample:
Jurkat (Human) Cell Lysate at 30 μg
K562 (Human) Cell Lysate at 30 μg
U87Mg (Human) Cell Lysate at 30 μg
Primary: Anti-caspase-8 subunit p18 (TMAB-00291) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55/18 kDa
Observed band size: 57 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 1μg /Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
Tissue SpecificityIsoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human caspase-8 subunit p18
Antigen Species
Human
Gene Name
CASP8
Gene ID
Protein Name
Caspase-8
Uniprot ID
Biology Area
Caspases,Metabolism,Necroptosis,Caspases,Caspases,Other proteases,Apoptosis
Function
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Chemical Properties
Molecular WeightTheoretical: 18/55 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-Caspase-8 subunit p18 Polyclonal Antibody | purchase Anti-Caspase-8 subunit p18 Polyclonal Antibody | Anti-Caspase-8 subunit p18 Polyclonal Antibody cost | order Anti-Caspase-8 subunit p18 Polyclonal Antibody | Anti-Caspase-8 subunit p18 Polyclonal Antibody molecular weight