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Anti-MST1 Polyclonal Antibody

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Catalog No. TMAB-01171
Alias YSK3, TIIAC, serine/threonine kinase 4, MST1, KRS2

Anti-MST1 Polyclonal Antibody is a Rabbit antibody targeting MST1. Anti-MST1 Polyclonal Antibody can be used in FCM,ICC/IF,IF,IHC-Fr,IHC-P.

Anti-MST1 Polyclonal Antibody

Anti-MST1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01171Alias YSK3, TIIAC, serine/threonine kinase 4, MST1, KRS2
Anti-MST1 Polyclonal Antibody is a Rabbit antibody targeting MST1. Anti-MST1 Polyclonal Antibody can be used in FCM,ICC/IF,IF,IHC-Fr,IHC-P.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-MST1 Polyclonal Antibody is a Rabbit antibody targeting MST1. Anti-MST1 Polyclonal Antibody can be used in FCM,ICC/IF,IF,IHC-Fr,IHC-P.
SynonymsYSK3, TIIAC, serine/threonine kinase 4, MST1, KRS2
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MST1) Polyclonal Antibody, Unconjugated (TMAB-01171) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MST1 Polyclonal Antibody, Unconjugated (TMAB-01171) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: Human laryngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MST1 Polyclonal Antibody, Unconjugated (TMAB-01171) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Tissue/cell: Human prostate tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MST1 Polyclonal Antibody, Unconjugated (TMAB-01171) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MST1) polyclonal Antibody, Unconjugated (TMAB-01171) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
6. Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-MST1 antibody (TMAB-01171)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:25; IF: 1:100-500; FCM: 2ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=The caspase-cleaved form cycles between the nucleus and cytoplasm.
Tissue SpecificityExpressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Ubiquitously expressed.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MST1 Cterminus
Antigen Species
Human
Gene Name
STK4
Gene ID
Protein Name
Serine/threonine-protein kinase 4
Uniprot ID
Biology Area
Apoptosis,Hypertrophy,Other Kinases
Function
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation (By similarity). Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes.
Chemical Properties
Molecular WeightTheoretical: 56 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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